在无感染性病毒情况下检测人扁桃体中的腺病毒核酸序列。

Detection of adenovirus nucleic acid sequences in human tonsils in the absence of infectious virus.

作者信息

Neumann R, Genersch E, Eggers H J

出版信息

Virus Res. 1987 Feb;7(1):93-7. doi: 10.1016/0168-1702(87)90060-8.

Abstract

Human tonsillar and adenoid tissues from surgical specimens and cell cultures established therefrom were screened for adenovirus type 2 (Ad2) sequences by in situ hybridization. For labeling we have utilized biotinylated DNA probes. We report detection of adenoviral sequences after hybridization with adenovirus type 2 DNA probes in tissues as well as in cell cultures from specimens without any signs of infectious virus even after long-term cultivation. In the infected tonsils only some of the cells appear to carry viral sequences. In conclusion, truly latent adenovirus infections in man seem to occur.

摘要

通过原位杂交技术，对手术标本中的人类扁桃体和腺样体组织以及由此建立的细胞培养物进行了2型腺病毒（Ad2）序列筛查。为进行标记，我们使用了生物素化的DNA探针。我们报告称，在用2型腺病毒DNA探针杂交后，在组织以及来自标本的细胞培养物中检测到了腺病毒序列，即使经过长期培养，这些标本也没有任何感染性病毒的迹象。在受感染的扁桃体中，只有部分细胞似乎携带病毒序列。总之，人类中似乎确实存在真正的腺病毒潜伏感染。