Gomes S A, Nascimento J P, Siqueira M M, Krawczuk M M, Pereira H G, Russell W C
J Virol Methods. 1985 Oct;12(1-2):105-10. doi: 10.1016/0166-0934(85)90012-6.
Adenovirus DNA was detected in cells from nasopharyngeal secretions of children with acute respiratory infections by in situ hybridization with biotinylated probes. The technique was easy to perform, giving rapid results which were well correlated with those of immunofluorescence assays of the same samples. Adenoviruses of subgroups B, C and E were detected equally well by probes prepared either from purified adenovirus type 5 or from a plasmid (A1) carrying a cloned insertion of BamH1 fragments C and D of adenovirus type 2 in pAT 153. The use of stable non-radioactive probes makes in situ hybridization a feasible assay for use in clinical laboratories with moderate resources.
通过用生物素化探针进行原位杂交，在患有急性呼吸道感染的儿童鼻咽分泌物细胞中检测到腺病毒DNA。该技术易于操作，结果快速，且与同一样本的免疫荧光测定结果高度相关。用从纯化的5型腺病毒或从携带腺病毒2型BamH1片段C和D克隆插入片段的质粒（A1）制备的探针，能同样良好地检测到B、C和E亚组的腺病毒。使用稳定的非放射性探针使原位杂交成为资源有限的临床实验室可行的检测方法。
