Faussone-Pellegrini M S
Acta Anat (Basel). 1987;128(2):98-109. doi: 10.1159/000146325.
Cytodifferentiation of the interstitial cells of Cajal related to the plexus entericus extremus and located on the inner face of the circular muscle layer of the mouse colon was studied in fetuses at term, unfed neonates, suckling and weaning young animals. In fetuses at term, the interstitial cells of Cajal are not found and their precursor cells are not identifiable among the cells present in the submucosal area facing the circular muscle layer, i.e., undifferentiated cells (mainly close to nerve fibers) and fibroblast-like cells (contacting each other and the developing smooth-muscle cells). Cells putatively considered as precursors of interstitial cells of Cajal, fibroblast-like cells rich in mitochondria, are present in unfed neonates (in small numbers) and in suckling animals (in large numbers). Differentiating interstitial cells of Cajal are undoubtedly recognizable during the second week of postnatal life (suckling period). Some of them have mixed fibroblastic- and interstitial-cell features and some have many interstitial-cell features. Both these cell types are already in contact with each other and differentiating and differentiated smooth-muscle cells. The undifferentiated cells, still present in unfed neonates, envelop the nerve fibers and differentiate as Schwann cells during the suckling period. During this period, as the nerve endings of the plexus entericus extremus develop, they immediately contact the differentiating interstitial cells of Cajal. During the weaning period, these cells are quite well differentiated although they do not have the same morphology as in adult animals before 30 days of age. Concomitantly, the nerve endings of the plexus entericus extremus contain an increasing number of synaptic vesicles.
研究了足月胎儿、未进食的新生儿、哺乳和断奶幼龄动物中，与肠末端神经丛相关且位于小鼠结肠环肌层内表面的Cajal间质细胞的细胞分化情况。在足月胎儿中，未发现Cajal间质细胞，在面向环肌层的黏膜下区域的细胞中，也无法识别其前体细胞，即未分化细胞（主要靠近神经纤维）和成纤维细胞样细胞（相互接触并与发育中的平滑肌细胞接触）。在未进食的新生儿（数量少）和哺乳动物（数量多）中，存在被认为可能是Cajal间质细胞前体的富含线粒体的成纤维细胞样细胞。在出生后第二周（哺乳期），无疑可以识别出正在分化的Cajal间质细胞。其中一些具有成纤维细胞和间质细胞的混合特征，一些具有许多间质细胞特征。这两种细胞类型已经相互接触，并与正在分化和已分化的平滑肌细胞接触。未分化细胞仍存在于未进食的新生儿中，包裹着神经纤维，并在哺乳期分化为雪旺细胞。在此期间，随着肠末端神经丛的神经末梢发育，它们立即与正在分化的Cajal间质细胞接触。在断奶期，这些细胞分化得相当好，尽管它们在30日龄前的形态与成年动物不同。与此同时，肠末端神经丛的神经末梢含有越来越多的突触小泡。
