College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China.
College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China.
Int J Biol Macromol. 2022 Jul 31;213:339-351. doi: 10.1016/j.ijbiomac.2022.05.173. Epub 2022 May 29.
This work investigated the protective effects of Cordyceps sinensis exopolysaccharide‑selenium nanoparticles (EPS-SeNPs) at different Se/EPS ratios (1/20, 1/3, 1/1, and 4/3) against 0.4 mM HO-induced oxidative stress in human hepatoma (HepG2) cells. Results revealed that EPS-SeNPs (0.1-4.0 μg/mL) promoted the proliferation of HepG2 cells with a cell viability of over 90% during HO stress. Different Se/EPS ratios of EPS-SeNPs provided protective effects against HO-induced oxidative stress in HepG2 cells by increasing cell viability, restoring cell and nucleus morphology, as well as reducing lactate dehydrogenase (LDH) levels. Particularly, EPS-SeNPs (Se/EPS = 1/1) with the smallest particle size showed the highest cell viability and the greatest inhibitory effect on LDH level. Besides, EPS-SeNPs also inhibited nitric oxide and reactive oxygen species (ROS) production, and increased mitochondrial membrane potential, superoxide dismutase (SOD), and catalase levels while reducing glutathione (GSH) content. Specially, EPS-SeNPs at Se/EPS ratio of 1/3 with smaller size showed the lowest ROS level and the highest antioxidant activities (SOD and GSH), implying that the ROS generation was inhibited by increasing enzymatic and non-enzymatic antioxidants. The enhanced protective effect of EPS-SeNPs (Se/EPS = 1/1 and 1/3) might be attributed to its smaller particle size.
本研究考察了不同硒/虫草多糖纳米硒比例（1/20、1/3、1/1 和 4/3）下蛹虫草胞外多糖-硒纳米颗粒（EPS-SeNPs）对 0.4mM 双氧水（HO）诱导的人肝癌（HepG2）细胞氧化应激的保护作用。结果表明，EPS-SeNPs（0.1-4.0μg/mL）在 HO 应激条件下可促进 HepG2 细胞增殖，细胞活力超过 90%。不同硒/虫草多糖纳米硒比例的 EPS-SeNPs 通过提高细胞活力、恢复细胞和细胞核形态以及降低乳酸脱氢酶（LDH）水平，对 HO 诱导的 HepG2 细胞氧化应激提供保护作用。特别是硒/虫草多糖纳米硒比例为 1/1、粒径最小的 EPS-SeNPs 表现出最高的细胞活力和对 LDH 水平的最大抑制作用。此外，EPS-SeNPs 还抑制一氧化氮和活性氧（ROS）的产生，增加线粒体膜电位、超氧化物歧化酶（SOD）和过氧化氢酶水平，同时降低谷胱甘肽（GSH）含量。特别是硒/虫草多糖纳米硒比例为 1/3、粒径较小的 EPS-SeNPs 表现出最低的 ROS 水平和最高的抗氧化活性（SOD 和 GSH），表明 ROS 的产生通过增加酶和非酶抗氧化剂而受到抑制。EPS-SeNPs（硒/虫草多糖纳米硒比例为 1/1 和 1/3）增强的保护作用可能归因于其较小的粒径。
