Integrated Laboratory of Pathogenic Biology, College of Preclinical Medicine, Dali University, Dali, 671000, People's Republic of China.
Department of Gastroenterology, Clinical Medical College and the First Affiliated Hospital of Chengdu Medical College, Chengdu, China.
Parasitol Res. 2022 Aug;121(8):2347-2358. doi: 10.1007/s00436-022-07561-0. Epub 2022 Jun 2.
Ticks are deemed to be second only to mosquitoes as the most common vector of human infectious diseases worldwide that give rise to human and animal diseases and economic losses to livestock production. Our understanding of the phylogenetic analysis between tick lineages has been restricted by the phylogenetic markers of individual genes. Genomic data research could help advance our understanding of phylogenetic analysis and molecular evolution. Mitochondrial genomic DNA facilitated the phylogenetic analysis of eukaryotes containing ticks. In this study, we sequenced and assembled the circular complete mitogenome information of Ixodes granulatus. The 14,540-bp mitogenome consists of 37 genes, including 13 protein-coding genes (PCGs), two genes for ribosomal RNA (rRNAs), and 22 genes for transfer RNA (tRNAs), and the origin of the L-strand replication region. The directions of the coding strand and component genes in the non-Australasian Ixodes mitochondrial genome were similar to those found in most other Australasian Ixodes, except for the loss of a lengthy control region. The phylogenetic tree based on maximum likelihood (ML) and Bayesian inference (BI) computational algorithms showed that I. granulatus exhibits a close relationship with I. hexagonus and I. ricinus. To our knowledge, this is the first study exploring the complete mitogenome for the species I. granulatus. Our results provide new insights for further research on the evolution, population genetics, systematics, and molecular ecology of ticks.
蜱虫被认为是仅次于蚊子的全球最常见的人类传染病传播媒介，可导致人类和动物疾病，并给畜牧业生产带来经济损失。我们对蜱虫谱系之间系统发育分析的理解受到了单个基因的系统发育标记的限制。基因组数据研究可以帮助我们深入了解系统发育分析和分子进化。线粒体基因组 DNA 促进了包含蜱虫的真核生物的系统发育分析。在这项研究中，我们对 Ixodes granulatus 的圆形完整线粒体基因组信息进行了测序和组装。该 14540bp 的线粒体基因组由 37 个基因组成，包括 13 个蛋白质编码基因（PCGs）、两个核糖体 RNA（rRNA）基因和 22 个转移 RNA（tRNA）基因，以及 L 链复制区的起点。非澳大拉西亚 Ixodes 线粒体基因组中编码链和组成基因的方向与大多数其他澳大拉西亚 Ixodes 的方向相似，除了失去一个长的控制区。基于最大似然法（ML）和贝叶斯推断（BI）计算算法构建的系统发育树表明，I. granulatus 与 I. hexagonus 和 I. ricinus 关系密切。据我们所知，这是首次对 I. granulatus 物种进行完整线粒体基因组研究。我们的研究结果为进一步研究蜱虫的进化、种群遗传学、系统学和分子生态学提供了新的见解。
