Liu Yafang, Chen Bin, Lu Xinyan, Liu Shuang, Jiang Dandan, Wang Xuan, Yi Lin, Li Rongyu, Zhang Quanfu, Wu Lixian, Yang Xing
Integrated Laboratory of Pathogenic Biology, College of Preclinical Medicine, Dali University, Dali, China.
School of Public Health, Dali University, Dali, China.
Front Vet Sci. 2023 Sep 7;10:1250381. doi: 10.3389/fvets.2023.1250381. eCollection 2023.
Fleas represent a group of paramount medical significance, subsisting on blood and acting as vectors for an array of naturally occurring diseases. These pathogens constitute essential elements within the plague biome, exerting deleterious effects on both human and livestock health. In this study, we successfully assembled and sequenced the whole mitochondrial genome of and using long-range PCR and next-generation sequencing technologies. The mitogenomes of and both have 37 genes with full lengths of 15,085 bp and 16,820 bp, respectively. The topology of the phylogenetic tree elucidates that species is clustered in a branch alongside other members of the family Leptopsyllidae, whereas species is a sister taxon to and . It also suggests that Pulicidae form a monophyletic clade, Ctenopthalmidae, Hystrichopsyllidae, Vermipsyllidae form a sister group to Ceratophyllidae/Leptopsyllidae group. The mitochondrial genomes of and were sequenced for the first time, which will contribute to a more comprehensive phylogenetic analysis of the Siphonaptera order. The foundation for subsequent systematic studies, and molecular biology of fleas was established.
跳蚤是一类具有极其重要医学意义的昆虫，以血液为食，并作为多种自然发生疾病的传播媒介。这些病原体是鼠疫生物群落的重要组成部分，对人类和牲畜健康都有有害影响。在本研究中，我们利用长片段PCR和新一代测序技术成功组装并测序了[两种跳蚤名称]的完整线粒体基因组。[两种跳蚤名称]的线粒体基因组均有37个基因，全长分别为15,085 bp和16,820 bp。系统发育树的拓扑结构表明，[一种跳蚤名称]与细蚤科的其他成员聚集在一个分支上，而[另一种跳蚤名称]是[相关跳蚤名称]和[相关跳蚤名称]的姐妹分类单元。它还表明，蚤科形成一个单系类群，栉眼蚤科、鬃眼蚤科、蠕形蚤科形成角叶蚤科/细蚤科类群的姐妹群。首次对[两种跳蚤名称]的线粒体基因组进行了测序，这将有助于对蚤目进行更全面的系统发育分析。为后续跳蚤的系统研究和分子生物学奠定了基础。
