Co-construction Collaborative Innovation Center for Chinese Medicine Resources Industrialization by Shaanxi & Education Ministry, State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi University of Chinese Medicine, Xianyang, PR China.
Co-construction Collaborative Innovation Center for Chinese Medicine Resources Industrialization by Shaanxi & Education Ministry, State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi University of Chinese Medicine, Xianyang, PR China; China Academy of Chinese Medical Sciences, Beijing 100700, PR China.
Food Res Int. 2022 Jun;156:111166. doi: 10.1016/j.foodres.2022.111166. Epub 2022 Mar 19.
To develop the medicinal and edible plant resources of Althaea officinalis Linn in Europe and other places, this study concentrated on the bioactive ingredients of its different extracts. The phytochemical compositions of MeOH extracts were evaluated by UPLC-DAD-ESI-Q-TOF-MS analysis. The in vitro antioxidant properties, enzymes inhibitory effects and nitric oxide (NO) production inhibitory activities of fractions obtained from the aerial parts of Althaea officinalis (APAO) were evaluated. The results identified 76 compounds, including 8 phenolic acids, 17 flavonoids, 6 coumarins, 9 triterpenes and 11 alkaloids. Fr. C-2 of APAO was found to have the highest TPC (175.8 ± 1.5 mg GAE/g) and TFC (466.9 ± 5.0 mg RE/g) with the highest antioxidant capacity in DPPH, ABTS, CUPRAC, FRAP and β-carotene bleaching assays. Fr. A showed noticeable inhibition of α-glucosidase with an IC value of 3.8 ± 0.1 μg/mL. However, Fr. B displayed stronger inhibitory activity on 5-lipoxygenase than quercetin, with the IC value of 8.4 ± 1.6 μg/mL. In addition, Fr. B also possessed potent inhibitory activities on NO production toward LPS-activated RAW 264.7 Cells with an IC value of 15.7 ± 1.6 μg/mL. Our findings suggest that different Althaea officinalis extracts may be considered sources of phenolic and flavonoid compounds with high potential as natural antioxidants, anti-inflammatory agents and blood sugar regulators. In addition, they can also be used in food and nutraceutical products with enhanced bioactivities.
为了开发欧洲和其他地方的药用和食用植物资源阿尔泰白芷（Althaea officinalis Linn），本研究集中于其不同提取物的生物活性成分。采用 UPLC-DAD-ESI-Q-TOF-MS 分析评估了 MeOH 提取物的植物化学成分。评估了从阿尔泰白芷（APAO）地上部分获得的馏分的体外抗氧化性能、酶抑制作用和一氧化氮（NO）产生抑制活性。结果鉴定出 76 种化合物，包括 8 种酚酸、17 种类黄酮、6 种香豆素、9 种三萜和 11 种生物碱。APAO 的 Fr. C-2 被发现具有最高的 TPC（175.8±1.5 mg GAE/g）和 TFC（466.9±5.0 mg RE/g），并且在 DPPH、ABTS、CUPRAC、FRAP 和 β-胡萝卜素漂白测定中具有最高的抗氧化能力。Fr. A 对 α-葡萄糖苷酶显示出显著的抑制作用，IC 值为 3.8±0.1 μg/mL。然而，Fr. B 对 5-脂氧合酶的抑制活性强于槲皮素，IC 值为 8.4±1.6 μg/mL。此外，Fr. B 对 LPS 激活的 RAW 264.7 细胞的 NO 产生也具有很强的抑制活性，IC 值为 15.7±1.6 μg/mL。我们的研究结果表明，不同的阿尔泰白芷提取物可能被认为是具有高潜力的酚类和类黄酮化合物的天然抗氧化剂、抗炎剂和血糖调节剂的来源。此外，它们还可以用于具有增强生物活性的食品和营养保健品中。
