Sichuan Agricultural University, Chengdu Campus, Chengdu 611130, China.
Sichuan Agricultural University, Chengdu Campus, Chengdu 611130, China; Animal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu 610066, Sichuan, China.
Food Res Int. 2022 Jun;156:111171. doi: 10.1016/j.foodres.2022.111171. Epub 2022 Mar 19.
To explore the chemical composition of chicken meat during different growth and development periods, the dynamic alterations of the metabolite composition were determined using LC-MS/MS-based metabolomics. Together, 573 metabolites were identified in chicken meat from five age stages. Generally, pentadecanoic acid, stearic acid, creatine, carnosine, IMP, L-histidine and L-isoleucine presented an upward trend with age, while anserine, DHA, L-aspartic acid, LPA 18:1 and LPI 18:1 decreased with age. The main pathways of chicken meat metabolism affected by age were fructose and mannose metabolism, arachidonic acid metabolism, steroid hormone biosynthesis, riboflavin metabolism, biosynthesis of unsaturated fatty acids, and linoleic acid metabolism. Using transcriptomic profiling data, we conducted Pearson correlation analysis between gene expression and metabolite profile data in each age comparison. Integration analysis of metabolome and transcriptome would be helpful to understand the biological processes underlying the development of meat quality and explore valuable biomarkers for specific metabolite accumulation.
为了探索不同生长发育阶段鸡肉中的化学成分，采用基于 LC-MS/MS 的代谢组学方法来确定代谢产物组成的动态变化。在五个不同年龄阶段的鸡肉中共鉴定出 573 种代谢产物。一般来说，随着年龄的增长，十五烷酸、硬脂酸、肌酸、肌肽、IMP、L-组氨酸和 L-异亮氨酸呈上升趋势，而鹅肌肽、二十二碳六烯酸、L-天冬氨酸、LPA 18:1 和 LPI 18:1 则随着年龄的增长而减少。受年龄影响的鸡肉代谢的主要途径是果糖和甘露糖代谢、花生四烯酸代谢、类固醇激素生物合成、核黄素代谢、不饱和脂肪酸的生物合成和亚油酸代谢。利用转录组学分析数据，我们对每个年龄比较中的基因表达和代谢物图谱数据进行 Pearson 相关性分析。代谢组学和转录组学的综合分析有助于了解肉质发育的生物学过程，并探索特定代谢产物积累的有价值的生物标志物。
