College of Animal Science, Zhejiang University, Hangzhou 310030, China.
J Agric Food Chem. 2024 Oct 2;72(39):21946-21956. doi: 10.1021/acs.jafc.4c05005. Epub 2024 Sep 18.
To explore the changes in meat quality and molecular mechanisms during the growth and development of Taihe black-bone silky fowl, this study employed liquid chromatography-mass spectrometry (LC-MS/MS) metabolomics to elucidate the dynamic changes of key differential metabolites (DMs) affecting meat quality, indicating that chicken at D120 had higher levels of ω-3 polyunsaturated fatty acids (PUFAs), creatine, anserine, and homocarnosine, while D150 had the most stachydrine and D210 had the most acylcarnitines. Additionally, D120 and D180 had more umami and sweet compounds. Furthermore, key metabolic pathways influenced by age included purine metabolism, the pentose phosphate pathway, nicotinate and nicotinamide metabolism, and taurine and hypotaurine metabolism. Transcriptomic identified differential expression genes (DEGs) are predominantly enriched in focal adhesion, the TGF-β signaling pathway, and the MAPK signaling pathway. Integrated metabolomics and transcriptomics revealed complex regulatory networks of DEGs and DMs in key metabolic pathways. This research enhanced our understanding of the biology of Taihe black-bone silky fowl meat quality, revealing possible biomarkers.
为了探究泰和乌鸡在生长发育过程中肉质的变化和分子机制，本研究采用液相色谱-质谱联用（LC-MS/MS）代谢组学方法阐明了影响肉质的关键差异代谢物（DMs）的动态变化，表明 D120 日龄鸡的 ω-3 多不饱和脂肪酸（PUFAs）、肌酸、鹅肌肽和同型瓜氨酸水平较高，而 D150 日龄鸡的獐牙菜苦苷含量最高，D210 日龄鸡的酰基辅酶 A 含量最高。此外，D120 日龄和 D180 日龄鸡的鲜味和甜味化合物含量更高。此外，受年龄影响的关键代谢途径包括嘌呤代谢、戊糖磷酸途径、烟酸和烟酰胺代谢以及牛磺酸和次牛磺酸代谢。转录组学鉴定的差异表达基因（DEGs）主要富集在粘着斑、TGF-β 信号通路和 MAPK 信号通路中。代谢组学和转录组学的整合揭示了关键代谢途径中 DEGs 和 DMs 的复杂调控网络。本研究增强了我们对泰和乌鸡肉质生物学的理解，揭示了可能的生物标志物。
