Wang Bingqian, Wang Songyang, Tang Yuqin, Jiang Lingli, He Wei, Lin Qinlu, Yu Feng, Wang Long
State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha, China.
National Engineering Laboratory for Rice and By-Product Deep Processing, Central South University of Forestry and Technology, Changsha, China.
Front Plant Sci. 2022 May 16;13:857390. doi: 10.3389/fpls.2022.857390. eCollection 2022.
Various long-lived mRNAs are stored in seeds, some of which are required for the initial phase of germination and are critical to seed longevity. However, the seed-specific long-lived mRNAs involved in seed longevity remain poorly understood in rice. To identify these mRNAs in seeds, we first performed aging experiment with 14 rice varieties, and categorized them as higher longevity (HL) and lower longevity (LL) rice varieties in conventional rice and hybrid rice, respectively. Second, RNA-seq analysis showed that most genes showed similar tendency of expression changes during natural and artificial aging, suggesting that the effects of these two aging methods on transcription are comparable. In addition, some differentially expressed genes (DEGs) in the HL and LL varieties differed after natural aging. Furthermore, several specific long-lived mRNAs were identified through a comparative analysis of HL and LL varieties after natural aging, and similar sequence features were also identified in the promoter of some specific long-lived mRNAs. Overall, we identified several specific long-lived mRNAs in rice, including gibberellin receptor gene , which may be associated with seed longevity.
多种长寿mRNA存储在种子中，其中一些是种子萌发初始阶段所必需的，并且对种子寿命至关重要。然而，在水稻中，参与种子寿命的种子特异性长寿mRNA仍知之甚少。为了鉴定种子中的这些mRNA，我们首先对14个水稻品种进行了老化实验，并分别将它们归类为常规水稻和杂交水稻中的高寿命（HL）和低寿命（LL）水稻品种。其次，RNA测序分析表明，大多数基因在自然老化和人工老化过程中表现出相似的表达变化趋势，这表明这两种老化方法对转录的影响具有可比性。此外，HL和LL品种中的一些差异表达基因（DEG）在自然老化后有所不同。此外，通过对自然老化后的HL和LL品种进行比较分析，鉴定出了几种特定的长寿mRNA，并且在一些特定长寿mRNA的启动子中也鉴定出了相似的序列特征。总体而言，我们在水稻中鉴定出了几种特定的长寿mRNA，包括赤霉素受体基因，其可能与种子寿命有关。
