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逆流线吸收免疫电泳是阿留申病(AD)根除计划中替代对流免疫电泳的一种诊断筛查试验。

Counter current line absorption immunoelectrophoresis in an alternative diagnostic screening test to counter current immunoelectrophoresis in Aleutian disease (AD) eradication programs.

作者信息

Aasted B, Alexandersen S, Cohn A, Hansen M

出版信息

Acta Vet Scand. 1986;27(3):410-20. doi: 10.1186/BF03548155.

Abstract

Counter current immunoelectrophoresis (CCIE) is the diagnostic method used in the ongoing Aleutian disease virus eradication program on Danish mink farms. There has been an increasing demand for an alternative diagnostic test especially to evaluate suspected false positive CCIE reactions. We compared test results of a number of negative and positive mink sera in indirect counter current immunoelectrophoresis (ICCIE), counter current line absorption immunoelectrophoresis (CCLAIE) and radio immuno assay (RIA) with test results from counter current immunoelectrophoresis and found that counter current line absorption immunoelectrophoresis is the best alternative diagnostic screening test to counter current immunoelectrophoresis for Aleutian disease eradication programs. Not only proved the CCLAIE test to be useful for evaluation of doubtfully positive CCIE reactions, but it was found to have a higher sensitivity than the CCIE test.

摘要

对流免疫电泳(CCIE)是丹麦水貂养殖场正在进行的阿留申病病毒根除计划中所使用的诊断方法。对于替代诊断测试的需求日益增加,尤其是用于评估疑似假阳性的CCIE反应。我们将间接对流免疫电泳(ICCIE)、对流线吸收免疫电泳(CCLAIE)和放射免疫分析(RIA)中一些阴性和阳性水貂血清的测试结果与对流免疫电泳的测试结果进行了比较,发现对流线吸收免疫电泳是阿留申病根除计划中对流免疫电泳的最佳替代诊断筛查测试。CCLAIE测试不仅被证明可用于评估可疑的阳性CCIE反应,而且还发现其灵敏度高于CCIE测试。

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