College of Chemistry and Molecular Sciences, Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan, China.
Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, China.
Angew Chem Int Ed Engl. 2022 Aug 8;61(32):e202207132. doi: 10.1002/anie.202207132. Epub 2022 Jun 24.
Microbial cell factories reinvigorate current industries by producing complex fine chemicals at low costs. Reduced nicotinamide adenine dinucleotide phosphate (NADPH) is the main reducing power to drive the biosynthetic pathways in microorganisms. However, insufficient intrinsic NADPH limits the productivity of microorganisms. Here, we report that supplying microorganisms with long-lived electrons from persistent phosphor mesoporous Al O (meso-Al O ) can elevate the NADPH level to facilitate efficient fine chemical production. The defects in meso-Al O were demonstrated to be highly efficient in prolonging electrons' lifetime. The long-lived electrons in meso-Al O can pass the material-microorganism interface and power the biosynthetic pathways of E. coli to produce jet fuel farnesene. This work represents a reliable strategy to design photo-biosynthesis systems to improve the productivity of microorganisms with solar energy.
微生物细胞工厂通过低成本生产复杂精细化学品而使当前产业重焕生机。还原型烟酰胺腺嘌呤二核苷酸磷酸（NADPH）是驱动微生物生物合成途径的主要还原力。然而，内在 NADPH 的不足限制了微生物的生产力。在这里，我们报告称，通过向微生物提供持久的磷介孔 Al2O3（meso-Al2O3）中的长寿命电子，可以提高 NADPH 水平，从而促进高效的精细化学品生产。证明介孔 Al2O3 中的缺陷在延长电子寿命方面非常有效。介孔 Al2O3 中的长寿命电子可以通过材料-微生物界面传递，并为大肠杆菌的生物合成途径提供动力，以生产喷气燃料法呢烯。这项工作代表了一种可靠的策略，用于设计光生物合成系统，以提高利用太阳能的微生物的生产力。
