CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.
School of Resources, Environment and Materials, Guangxi University, Nanning, 530004, China.
Adv Sci (Weinh). 2024 Jul;11(25):e2308597. doi: 10.1002/advs.202308597. Epub 2024 Apr 25.
The development of semi-artificial photosynthetic systems, which integrate metal-organic frameworks (MOFs) with industrial microbial cell factories for light-driven synthesis of fuels and valuable chemicals, represents a highly promising avenue for both research advancements and practical applications. In this study, an MOF (PCN-222) utilizing racemic-(4-carboxyphenyl) porphyrin and zirconium chloride (ZrCl) as primary constituents is synthesized. Employing a self-assembly process, a hybrid system is constructed, integrating engineered Escherichia coli (E. coli) to investigate light-driven hydrogen and lysine production. These results demonstrate that the light-irradiated biohybrid system efficiently produce H with a quantum efficiency of 0.75% under full spectrum illumination, the elevated intracellular reducing power NADPH is also observed. By optimizing the conditions, the biohybrid system achieves a maximum lysine production of 18.25 mg L, surpassing that of pure bacteria by 332%. Further investigations into interfacial electron transfer mechanisms reveals that PCN-222 efficiently captures light and facilitates the transfer of photo-generated electrons into E. coli cells. It is proposed that the interfacial energy transfer process is mediated by riboflavin, with facilitation by secreted small organic acids acting as hole scavengers for PCN-222. This study establishes a crucial foundation for future research into the light-driven biomanufacturing using E. coli-based hybrid systems.
半人工光合系统的发展，将金属-有机骨架（MOFs）与工业微生物细胞工厂相结合，用于光驱动合成燃料和有价值的化学品，为研究进展和实际应用提供了一条极具前景的途径。在这项研究中，合成了一种利用外消旋-（4-羧基苯基）卟啉和氯化锆（ZrCl）作为主要成分的 MOF（PCN-222）。通过自组装过程，构建了一个集成工程大肠杆菌（E. coli）的混合系统，用于研究光驱动氢气和赖氨酸的生产。这些结果表明，在全光谱照射下，光辐照生物混合系统能够高效地生产 H，量子效率为 0.75%，同时观察到细胞内还原力 NADPH 的升高。通过优化条件，生物混合系统实现了 18.25mg L 的最大赖氨酸产量，比纯细菌提高了 332%。进一步研究界面电子转移机制表明，PCN-222 能够有效地捕获光，并促进光生电子向 E. coli 细胞的转移。据推测，界面能量转移过程是由核黄素介导的，分泌的小分子有机酸作为 PCN-222 的空穴清除剂促进了这一过程。这项研究为未来使用基于大肠杆菌的混合系统进行光驱动生物制造的研究奠定了重要基础。
