Department of Mechanical Engineering, University College London, London, UK.
Antwerp Surgical Training, Anatomy and Research Centre (ASTARC), University of Antwerp, Wilrijk, Belgium.
Sci Data. 2022 Jun 2;9(1):264. doi: 10.1038/s41597-022-01353-y.
Technological advancements in X-ray imaging using bright and coherent synchrotron sources now allows the decoupling of sample size and resolution while maintaining high sensitivity to the microstructures of soft, partially dehydrated tissues. The continuous developments in multiscale X-ray imaging resulted in hierarchical phase-contrast tomography, a comprehensive approach to address the challenge of organ-scale (up to tens of centimeters) soft tissue imaging with resolution and sensitivity down to the cellular level. Using this technique, we imaged ex vivo an entire human left lung at an isotropic voxel size of 25.08 μm along with local zooms down to 6.05-6.5 μm and 2.45-2.5 μm in voxel size. The high tissue contrast offered by the fourth-generation synchrotron source at the European Synchrotron Radiation Facility reveals the complex multiscale anatomical constitution of the human lung from the macroscopic (centimeter) down to the microscopic (micrometer) scale. The dataset provides comprehensive organ-scale 3D information of the secondary pulmonary lobules and delineates the microstructure of lung nodules with unprecedented detail.
利用明亮且相干的同步辐射源的 X 射线成像技术的进步，现在可以在保持对软、部分脱水组织微观结构的高灵敏度的同时，解耦样品尺寸和分辨率。多尺度 X 射线成像的不断发展导致了分级相衬断层摄影术的出现，这是一种全面的方法，可以解决器官尺度（可达数十厘米）软组织成像的挑战，分辨率和灵敏度可达到细胞水平。使用这种技术，我们对整个人体左肺进行了离体成像，体素大小为 25.08μm，局部放大倍数分别为 6.05-6.5μm 和 2.45-2.5μm。欧洲同步辐射装置第四代同步辐射源提供的高组织对比度揭示了人体肺从宏观（厘米）到微观（微米）尺度的复杂多尺度解剖结构。该数据集提供了次级肺小叶的全面器官尺度 3D 信息，并以前所未有的细节描绘了肺结节的微观结构。
