Li Dan, Ding Shijie, Luo Manting, Chen Jinguo, Zhang Qiukun, Liu Yijuan, Li Anlan, Zhong Shuncong, Ding Jian
Digestive Department, Union Hospital, Fujian Medical University, Fuzhou, China.
Digestive Department, the First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
Quant Imaging Med Surg. 2022 Jun;12(6):3193-3203. doi: 10.21037/qims-21-1062.
The differential diagnosis of acute and chronic colitis remains a common clinical problem. Optical coherence tomography (OCT) is a non-invasive, high-resolution imaging technique that can be used to measure morphological changes in the intestinal wall and estimate intestinal inflammation. We aimed to conduct an experiment on a mouse model investigate the value of OCT as a tool for the differential diagnosis of acute and chronic colitis.
Mice were administered dextran sulfate sodium salt (DSS) to construct acute and chronic colitis models. Acutely- and chronically-affected intestinal walls were scanned by OCT, and then the scanned colonic tissue samples were stained with hematoxylin and eosin (HE). Structural and morphological changes indicating inflammation in the intestinal wall were evaluated in the HE sections and OCT images using different parameters. The parameters were used in one-way analysis of variance (ANOVA) to screen for a differential diagnosis of acute or chronic colitis.
For the HE sections, the angle of the mucosal folds, length of the basilar part, and submucosal height and area were statistically significant parameters in the comparisons between the mice with acute colitis and the control-group mice (P<0.05). In the comparisons between chronic colitis mice and control-group mice, the angle of the mucosal folds, length of the basilar part, submucosal height and area, muscularis thickness, submucosal height + muscularis thickness, and mucosal thickness were statistically significant parameters (P<0.05). Finally, in the comparisons between acute colitis mice and those with chronic colitis, the angle of the mucosal folds, submucosal height and area, muscularis thickness, submucosal height + muscularis thickness, and mucosal thickness were statistically significant parameters (P<0.05). For the OCT images, only the length of the basilar part and submucosal height + muscularis thickness were statistically significant parameters between the acute colitis mice and control-group mice (P<0.05). The length of the basilar part and submucosal height + muscularis thickness were statistically significant between chronic colitis mice and control-group mice (P<0.05). In the comparisons between acute colitis mice and those with chronic colitis, only submucosal height + muscularis thickness was a statistically significant parameter (P<0.05).
Certain intestinal wall parameters in OCT can be used to make a differential diagnosis between acute and chronic colitis possible. This study contributes to constructing a potential diagnostic system for evaluating colorectal inflammation using OCT.
急性和慢性结肠炎的鉴别诊断仍是常见的临床问题。光学相干断层扫描（OCT）是一种非侵入性的高分辨率成像技术，可用于测量肠壁的形态变化并评估肠道炎症。我们旨在通过对小鼠模型进行实验，研究OCT作为急性和慢性结肠炎鉴别诊断工具的价值。
给小鼠施用葡聚糖硫酸钠（DSS）以构建急性和慢性结肠炎模型。用OCT扫描急性和慢性病变的肠壁，然后将扫描的结肠组织样本用苏木精和伊红（HE）染色。使用不同参数在HE切片和OCT图像中评估表明肠壁炎症的结构和形态变化。这些参数用于单因素方差分析（ANOVA）以筛选急性或慢性结肠炎的鉴别诊断。
对于HE切片，在急性结肠炎小鼠与对照组小鼠的比较中，黏膜皱襞角度、基底部分长度、黏膜下层高度和面积是具有统计学意义的参数（P<0.05）。在慢性结肠炎小鼠与对照组小鼠的比较中，黏膜皱襞角度、基底部分长度、黏膜下层高度和面积、肌层厚度、黏膜下层高度+肌层厚度以及黏膜厚度是具有统计学意义的参数（P<0.05）。最后，在急性结肠炎小鼠与慢性结肠炎小鼠的比较中，黏膜皱襞角度、黏膜下层高度和面积、肌层厚度、黏膜下层高度+肌层厚度以及黏膜厚度是具有统计学意义的参数（P<0.05）。对于OCT图像，仅基底部分长度和黏膜下层高度+肌层厚度在急性结肠炎小鼠与对照组小鼠之间是具有统计学意义的参数（P<0.05）。基底部分长度和黏膜下层高度+肌层厚度在慢性结肠炎小鼠与对照组小鼠之间具有统计学意义（P<0.05）。在急性结肠炎小鼠与慢性结肠炎小鼠的比较中，仅黏膜下层高度+肌层厚度是具有统计学意义的参数（P<0.05）。
OCT中的某些肠壁参数可用于实现急性和慢性结肠炎的鉴别诊断。本研究有助于构建一个使用OCT评估结肠直肠炎症的潜在诊断系统。
