Ding Jian, Lin Jiewen, Li Qiu, Chen Xiaoping, Chen Weiqiang, Zhang Qiukun, He Shanshan, Wu Ting, Wang Chengdang, Zhong Shuncong, Li Dan
Digestive Department, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, China.
Laboratory of Optics, Terahertz and Nondestructive Testing, School of Mechanical Engineering and Automation, Fuzhou University, Fuzhou 350108, China.
Quant Imaging Med Surg. 2020 May;10(5):945-957. doi: 10.21037/qims.2020.04.04.
There is an urgent need to develop a noninvasive imaging technique for the diagnosis of early inflammatory lesions or early and real-time microscopic assessment before selecting the most representative biopsy sites.
In this study, a dextran sulfate sodium colitis model was developed, and intestinal histological damage scores measured the degree of inflammation in colitis. According to these scores, 6 parameters were designed for hematoxylin and eosin (HE) sections based on morphological changes, and 2 parameters were designed for optical coherence tomography (OCT) images to measure submucosal edema by morphological changes to evaluate inflammation degrees in the colon. Spearman's rank correlation method was used to compare the correlation between the submucosal morphological changes and the different degrees of inflammation. One-way analysis of variance (ANOVA) was used for comparisons among groups, while receiver operating characteristic (ROC) curves of the indicators in HE sections and OCT images were plotted.
In HE sections, angle of mucosal folds (r=0.853, P<0.01), length of basilar parts (r=0.915, P<0.01), submucosal area (r=0.819, P<0.01), and height between submucosal and muscular layers (r=0.451, P=0.001) were correlated with the degree of inflammation in colitis. In OCT images, length of basilar parts (r=0.800, P<0.01) and height of submucosa + thickness of muscularis (r=0.648, P=0.001) were correlated with the degree of inflammation and aided the measurement of inflammation in the colon.
Parameters based on morphological changes in OCT images and HE sections were significant indexes for evaluating the degree of inflammation in colitis. OCT images have advantages for future clinical applications in situ, including noninvasiveness and real-time imaging.
迫切需要开发一种非侵入性成像技术，用于在选择最具代表性的活检部位之前诊断早期炎症性病变或进行早期和实时的微观评估。
在本研究中，建立了葡聚糖硫酸钠结肠炎模型，通过肠道组织学损伤评分来衡量结肠炎的炎症程度。根据这些评分，基于苏木精和伊红（HE）切片的形态学变化设计了6个参数，基于光学相干断层扫描（OCT）图像的形态学变化设计了2个参数，以测量黏膜下层水肿来评估结肠炎症程度。采用Spearman等级相关法比较黏膜下层形态学变化与不同程度炎症之间的相关性。采用单因素方差分析（ANOVA）进行组间比较，同时绘制HE切片和OCT图像中各项指标的受试者工作特征（ROC）曲线。
在HE切片中，黏膜皱襞角度（r = 0.853，P < 0.01）、基底部分长度（r = 0.915，P < 0.01）、黏膜下层面积（r = 0.819，P < 0.01）以及黏膜下层与肌层之间的高度（r = 0.451，P = 0.001）与结肠炎炎症程度相关。在OCT图像中，基底部分长度（r = 0.800，P < 0.01）和黏膜下层高度 + 肌层厚度（r = 0.648，P = 0.001）与炎症程度相关，并有助于测量结肠炎症。
基于OCT图像和HE切片形态学变化的参数是评估结肠炎炎症程度的重要指标。OCT图像在未来临床原位应用中具有优势，包括非侵入性和实时成像。
