Center for Education and Research on Dental Implants, Post Graduation Program in Dentistry, Department of Dentistry, Federal University of Santa Catarina, Florianopolis, Santa Catarina, Brazil, e-mail:
Centro de Pesquisa São Leopoldo Mandic, R Dr José Rocha Junqueira, Campinas, São Paulo, Brazil.
J Contemp Dent Pract. 2022 Jan 1;23(1):22-30.
The objective of this study was to evaluate the viability and morphology of human fibroblasts and keratinocytes cells, both grown on stainless steel (steel) (18Cr14Ni2.5Mo), and polyether-ether-ketone (PEEK) surfaces, hypothesizing the use of these surfaces as novel materials for prosthetic components.
Gingival human keratinocytes and gingival human fibroblasts lines were grown on discs made by steel ( = 36), PEEK ( = 36), and titanium (Ti) (Ti6A14V) ( = 36)-control. For viability assay, cultures were grown at 24 hours (TV1), 48 hours (TV2), and 72 hours (TV3) times and evaluated by the colorimetric tetrazolium assay (MTT). For morphology and cell adhesion assays, after 24 hours (TM1), 48 hours (TM2), and 96 hours (TM3) of cell culture, cells were examined by scanning electron microscopy (SEM) and analyzed at magnifications with 500×, 1,000×, and 2,500×.
Regarding the viability, the keratinocytes did not present statistical difference on the different materials, in TV1 and TV3 times of culture. Their growth rate increased on all materials, being more expressive in steel; the fibroblasts did not present statistical difference on the different materials, in TV2 and TV3 times of culture. The growth rate of these decreased on all materials, being more expressive in PEEK. The morphology analyses show increase in cell numbers, adequate spreading, and adhesion at all cultivation times (TM1, TM2, and TM3) in both cell lines, on all materials.
All materials tested are suitable for use in the manufacture of prosthetic components for implant-supported rehabilitations, considering the limitations of this study.
This work analyzes the cellular response of cells present in the human gingiva, as a way to simulate the peri-implant tissue response around novel angular prosthetic components made of stainless steel and PEEK.
本研究旨在评估人成纤维细胞和角质形成细胞在不锈钢（钢）（18Cr14Ni2.5Mo）和聚醚醚酮（PEEK）表面上的活力和形态，假设这些表面可作为新型义齿修复体组件材料。
将牙龈人角质形成细胞和牙龈人成纤维细胞系生长在由钢（n = 36）、PEEK（n = 36）和钛（Ti）（Ti6A14V）（n = 36）-对照制成的圆盘上。为了进行活力测定，将培养物在 24 小时（TV1）、48 小时（TV2）和 72 小时（TV3）时间生长，并通过比色四唑测定法（MTT）进行评估。为了进行形态和细胞黏附测定，在细胞培养 24 小时（TM1）、48 小时（TM2）和 96 小时（TM3）后，通过扫描电子显微镜（SEM）检查细胞，并在 500×、1,000×和 2,500×放大倍数下进行分析。
关于活力，角质形成细胞在 TV1 和 TV3 培养时间的不同材料上没有统计学差异。它们在所有材料上的生长速度均增加，在钢上更为明显；成纤维细胞在 TV2 和 TV3 培养时间的不同材料上没有统计学差异。这些细胞在所有材料上的生长速度均降低，在 PEEK 上更为明显。形态分析表明，在所有培养时间（TM1、TM2 和 TM3），两种细胞系在所有材料上的细胞数量均增加，铺展和黏附良好。
在考虑到这项研究的局限性的情况下，所有测试的材料均适合用于制造用于植入物支持修复的义齿组件。
本工作分析了存在于人类牙龈中的细胞的细胞反应，作为模拟新型角状义齿修复体组件周围的种植体周围组织反应的一种方式。
