采用高效液相色谱-真空紫外电感耦合等离子体原子发射光谱法（HPLC-ICP）检测对照大鼠和镉暴露大鼠器官中的含硫化合物。

Detection of sulfur-containing compounds in control and cadmium-exposed rat organs by high-performance liquid chromatography--vacuum-ultraviolet inductively coupled plasma-atomic emission spectrometry (HPLC-ICP).

作者信息

Sunaga H, Kobayashi E, Shimojo N, Suzuki K T

出版信息

Anal Biochem. 1987 Jan;160(1):160-8. doi: 10.1016/0003-2697(87)90626-9.

Abstract

Sulfur-containing compounds in biological samples were separated by high-performance gel permeation chromatography and detected with a vacuum-ultraviolet inductively coupled plasma-atomic emission spectrometer. Distribution profiles of sulfur in the supernatants of liver, kidney, spleen, lung, and pancreas of control and cadmium-exposed rats were determined along with cadmium, copper, iron, phosphorus, and zinc profiles. Changes in sulfur distribution were induced by cadmium exposure not only in the metallothionein fraction, but also in the high-molecular-weight protein fraction, indicating the effect of cadmium exposure on diverse endogenous sulfur-containing compounds. Glutathione and taurine also were detected simultaneously as distinct peaks.

摘要

生物样品中的含硫化合物通过高效凝胶渗透色谱法进行分离，并用真空紫外电感耦合等离子体原子发射光谱仪进行检测。测定了对照大鼠和镉暴露大鼠肝脏、肾脏、脾脏、肺和胰腺上清液中硫的分布情况，以及镉、铜、铁、磷和锌的分布情况。镉暴露不仅在金属硫蛋白组分中，而且在高分子量蛋白质组分中诱导了硫分布的变化，表明镉暴露对多种内源性含硫化合物有影响。同时还检测到谷胱甘肽和牛磺酸为明显的峰。