Suzuki K T, Sunaga H, Kobayashi E, Shimojo N
Toxicol Appl Pharmacol. 1986 Dec;86(3):466-73. doi: 10.1016/0041-008x(86)90374-1.
Cadmium-binding proteins in blood serum were determined on a gel permeation column by high-performance liquid chromatography-inductively coupled argon plasma-atomic emission spectrometry. Cadmium chloride was administered iv to female rats of the Wistar strain in a single dose of 0.4 mg Cd/kg body wt and the rats were killed 1, 2, 3, 5, 10, 20, and 30 min after the injection. The blood serum was separated on an Asahipak GS-520 column and cadmium concentration in the eluate was monitored continuously along with sulfur, zinc, copper, iron, and phosphorus concentrations. Cadmium was selectively bound to mercaptalbumin and the metal was eliminated from blood serum within 30 min in a biphasic mode. Addition in vitro of cadmium chloride into fresh blood serum revealed that cadmium is bound selectively to mercaptalbumin up to approximately 14 micrograms Cd/ml serum. Excess cadmium in blood serum was found in the lower molecular weight fraction. Nonmercaptalbumin produced by oxidative disulfide bond formation between albumin and glutathione or cysteine was not able to bind cadmium. Sulfur and other element profiles were helpful in characterizing metal-binding proteins.
采用高效液相色谱-电感耦合氩等离子体-原子发射光谱法，在凝胶渗透柱上测定血清中的镉结合蛋白。以0.4mg Cd/kg体重的单剂量静脉注射氯化镉给Wistar品系的雌性大鼠，在注射后1、2、3、5、10、20和30分钟处死大鼠。血清在Asahipak GS-520柱上分离，同时连续监测洗脱液中镉浓度以及硫、锌、铜、铁和磷的浓度。镉选择性地与巯基白蛋白结合，并且该金属在30分钟内以双相模式从血清中消除。在新鲜血清中体外添加氯化镉表明，镉选择性地与巯基白蛋白结合，直至血清中镉浓度约为14μg/ml。血清中过量的镉存在于较低分子量部分。由白蛋白与谷胱甘肽或半胱氨酸之间形成氧化二硫键产生的非巯基白蛋白不能结合镉。硫和其他元素谱有助于表征金属结合蛋白。
