从巴基斯坦鸡肉中分离和分子特征分析产超广谱β内酰胺酶的大肠杆菌。

Isolation and molecular characterization of extended spectrum beta lactamase producing Escherichia coli from chicken meat in Pakistan.

机构信息

Centre of Biotechnology and Microbiology, University of Peshawar, Peshawar, Pakistan.

Department of Biological Sciences, Faculty of Science, King Abdul Aziz University, Jeddah, Saudi Arabia.

出版信息

PLoS One. 2022 Jun 3;17(6):e0269194. doi: 10.1371/journal.pone.0269194. eCollection 2022.

The goal of this study was to find E. coli, a prevalent pathogen that causes food-borne illnesses, in chicken samples (n = 500) collected from three districts in KhyberPukhtunkhwa: Mardan, Swabi, and Swat. The E. coli isolates were identified by Gram staining, API strips and Universal Stress Protein. A total of 412 samples tested positive for E. coli and were sensitive to MEM, TZP, and FOS as evidenced by disc diffusion method. The isolates were resistant to TE, NOR, and NA with statistically significant results (P≤0.05). The isolates showed the presence of different antibiotic resistance genes; blaOXA-1, blaCTX-M15, blaTEM-1, QnrS, TetA, AAC, AAD, Sul1 and Sul2. The results revealed mutations in blaOXA-1 gene (H81Q), blaTEM-1 (C108Y, T214A, K284E and P301S), QnrS (H95R) and Sul2 (E66A). The findings of this study may be helpful in better management of E. coli infections by physicians.

本研究的目的是在从开伯尔-普赫图赫瓦省三个地区（马尔丹、斯瓦比和斯瓦特）采集的 500 份鸡样本中发现大肠杆菌，这是一种常见的食源性病原体。通过革兰氏染色、API 条带和普遍应激蛋白鉴定大肠杆菌分离株。总共 412 个样本通过纸片扩散法检测到对 MEM、TZP 和 FOS 敏感，呈大肠杆菌阳性。分离株对 TE、NOR 和 NA 具有耐药性，结果具有统计学意义（P≤0.05）。分离株显示存在不同的抗生素耐药基因；blaOXA-1、blaCTX-M15、blaTEM-1、QnrS、TetA、AAC、AAD、Sul1 和 Sul2。结果表明 blaOXA-1 基因（H81Q）、blaTEM-1（C108Y、T214A、K284E 和 P301S）、QnrS（H95R）和 Sul2（E66A）发生突变。本研究的结果可能有助于医生更好地管理大肠杆菌感染。