Department of Botany, Mizoram University, Aizawl 796004, Mizoram, India.
Dept. of Horticulture, Aromatic and Medicinal Plants, India.
Biomed Pharmacother. 2022 Jun;150:113039. doi: 10.1016/j.biopha.2022.113039. Epub 2022 May 12.
The aim of the current study was to examine the efficacy of the leaf, stem and rhizome of Curcuma aeruginosa Roxb. for their phytochemical content, antioxidant and anti-cancer activities. The different parts of C. aeruginosa were subjected to sequential extraction to give three fractions viz., hexane, ethyl acetate and methanol extract. The cytotoxic effect and the mode of action against A-549 human lung adenocarcinoma and HeLa cell lines were examined. C. aeruginosa presented no significant toxic effect in normal human lung cells (L-132). The methanol extracts were found to be the most cytotoxic and further investigation was carried out to understand the effects. The methanol extracts induced DNA damage after 24 h with significant increase in tail DNA and tail moment when compared to untreated control. Up-regulation in the expression of the caspase - 8 and - 3 activity was observed after 48 h of treatment. The mechanism of cell death and apoptosis induced by the methanol extracts on A549 and HeLa cells were studied using fluorescent staining. Bioactive compounds detected from the HPLC revealed phenol and flavonoid compounds: Gallic acid, quercetin, caffeic acid, kaempferol, rutin, coumaric acid and naringenin. GC-MS results identified the presence of sesquiterpenoids: α-curcumene, curzerene curcumenol, curzerenone epicurzerenone, caryophyllene oxide and diterpenoid, andrographolide. These compounds are known for inducing apoptosis in human cancer cells through caspase - dependent pathways. Therefore, C. aeruginosa and its potential to induce apoptosis in cancer cells suggest that they have potential in medical applications.
本研究旨在考察莪术叶、茎和根茎的化学成分、抗氧化和抗癌活性。将莪术的不同部位进行连续提取，得到三种提取物：正己烷、乙酸乙酯和甲醇提取物。检测了莪术不同部位提取物对 A-549 人肺腺癌细胞和 HeLa 细胞系的细胞毒性作用和作用方式。莪术对正常人体肺细胞（L-132）没有显著的毒性作用。甲醇提取物的细胞毒性最强，进一步的研究表明，甲醇提取物在 24 小时后诱导 DNA 损伤，与未经处理的对照组相比，尾部 DNA 和尾部矩显著增加。与未经处理的对照组相比，48 小时处理后观察到 caspase-8 和 caspase-3 活性的表达上调。通过荧光染色研究了甲醇提取物对 A549 和 HeLa 细胞的细胞死亡和凋亡诱导的机制。HPLC 检测到的生物活性化合物包括酚类和类黄酮化合物：没食子酸、槲皮素、咖啡酸、山奈酚、芦丁、香豆酸和柚皮苷。GC-MS 结果鉴定出倍半萜：α-姜黄烯、莪术烯、莪术醇、莪术酮、莪术酮、菖蒲烯氧化物和二萜、穿心莲内酯。这些化合物通过半胱天冬酶依赖性途径诱导人癌细胞凋亡。因此，莪术及其诱导癌细胞凋亡的潜力表明它在医学应用中有潜在的应用价值。
