Department of General Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 3 East Qingchun Road, Hangzhou, 310016, Zhejiang, China.
J Transl Med. 2022 Jun 3;20(1):250. doi: 10.1186/s12967-022-03411-w.
Gastric cancer is a common type of gastrointestinal malignant tumor in China. The mechanism of the development and progression of gastric cancer remains the continuing research focus. The tumor microenvironment plays an important role in the development and progression of tumors. The present study used single-cell sequencing data to characterize the microenvironment of gastric cancer, investigate the effects of oxidative stress on gastric cancer microenvironmental cells through the comparison between cancer tissue and normal tissue, and identify the key genes associated with gastric cancer patients' survival. The results showed that compared with normal gastric tissue, gastric cancer tissue had a decreased oxidative stress response, weaker oxidative detoxification ability, and increased oxidative stress-induced cell death. In the different types of single cells of gastric cancer microenvironment, the oxidative stress response of T cell was increased, the ability of oxidative detoxification was enhanced, and the oxidative stress-induced cell death was exacerbate. Mucous cell showed the same trend as gastric cancer cells: decreased oxidative stress response, weak oxidative detoxification ability, and weakened oxidative stress-induced cell death. Moreover, TRIM62, MET, and HBA1, which were significantly associated with oxidative stress, may be biomarkers for the prognosis of gastric cancer. High expression of TRIM62 indicated a good prognosis, while MET and HBA1 indicated a poor prognosis, which will be confirmed by further clinical studies.
胃癌是中国常见的一种胃肠道恶性肿瘤。胃癌的发生发展机制仍然是持续研究的重点。肿瘤微环境在肿瘤的发生发展中起重要作用。本研究使用单细胞测序数据对胃癌的微环境进行了特征描述，通过比较癌组织和正常组织，研究了氧化应激对胃癌微环境细胞的影响，并鉴定了与胃癌患者生存相关的关键基因。结果表明，与正常胃组织相比，胃癌组织的氧化应激反应减弱，氧化解毒能力较弱，氧化应激诱导的细胞死亡增加。在胃癌微环境的不同类型的单细胞中，T 细胞的氧化应激反应增强，氧化解毒能力增强，氧化应激诱导的细胞死亡加剧。黏液细胞与胃癌细胞表现出相同的趋势：氧化应激反应减弱，氧化解毒能力较弱，氧化应激诱导的细胞死亡减弱。此外，与氧化应激显著相关的 TRIM62、MET 和 HBA1 可能是胃癌预后的标志物。TRIM62 表达水平高提示预后良好，而 MET 和 HBA1 表达水平高提示预后不良，这将通过进一步的临床研究得到证实。
