Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, P.O. Box 46414-356, Noor, Iran; Bioactive Compounds Group, Faculty of Interdisciplinary Sciences and Technologies, Tarbiat Modares University, P.O. Box 14115-336, Tehran, Iran.
Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, P.O. Box 46414-356, Noor, Iran.
Int J Biol Macromol. 2022 Jul 31;213:546-554. doi: 10.1016/j.ijbiomac.2022.05.174. Epub 2022 Jun 2.
Polysaccharides from Pimpinella anisum were isolated using water at elevated temperature and DEAE Sepharose FF chromatography to examine their chemical structure and activation capacity on immune cells. P. anisum fractions (PAF1, PAF2 and PAF3) were mainly composed of neutral sugars (84.0-98.2%) and uronic acids (2.1-11.8%) with weight average molecular weight (M) ranging from 186.6 to 5474.5 × 10 g/mol. Polysaccharides induced a significant inflammatory response in RAW264.7 murine macrophage cells releasing nitric oxide and expressing TNF-α, IL-1β, IL-6 and IL-10 cytokines. The induction of NK-92 natural killer cells resulted in TNF-α and IFN-γ production and activation of GrB/perforin-, NKG2D- and FasL-mediated cytotoxicity. Polysaccharides triggered the phosphorylation of NF-κB, ERK, JNK and p38 proteins in RAW264.7 and NK-92 cells indicating the involvement of NF-κB and MAPKs signaling pathways. The most active polysaccharide was a galactoarabinan with complex structure.
从莳萝中分离出的多糖采用水在高温和 DEAE Sepharose FF 层析法来研究其化学结构和对免疫细胞的激活能力。莳萝部分（PAF1、PAF2 和 PAF3）主要由中性糖（84.0-98.2%）和糖醛酸（2.1-11.8%）组成，重均分子量（M）范围为 186.6 至 5474.5×10g/mol。多糖在 RAW264.7 鼠巨噬细胞中诱导显著的炎症反应，释放一氧化氮并表达 TNF-α、IL-1β、IL-6 和 IL-10 细胞因子。NK-92 自然杀伤细胞的诱导导致 TNF-α 和 IFN-γ 的产生以及 GrB/穿孔素、NKG2D 和 FasL 介导的细胞毒性的激活。多糖触发了 RAW264.7 和 NK-92 细胞中 NF-κB、ERK、JNK 和 p38 蛋白的磷酸化，表明 NF-κB 和 MAPKs 信号通路的参与。最活跃的多糖是一种具有复杂结构的半乳阿拉伯聚糖。
