从接骨木镰刀菌NRRL 13495培养物中制备4,15-二乙酰氧基镰刀菌烯醇
Preparation of 4,15-diacetoxyscirpenol from cultures of Fusarium sambucinum NRRL 13495.
作者信息
Richardson K E, Hamilton P B
出版信息
Appl Environ Microbiol. 1987 Feb;53(2):460-2. doi: 10.1128/aem.53.2.460-462.1987.
Abstract
Filtrates of Fusarium sambucinum NRRL 13495 grown in a stagnant culture for 9 days contained up to 458 +/- 60 (mean +/- standard error; n = 3) mg of 4,15-diacetoxyscirpenol per liter depending on culture conditions. Extraction with ethyl acetate, chromatography on a column of silica gel, and crystallization from mixtures of ethyl acetate and hexane provided pure material in 96% yield.
摘要
在静止培养9天的接骨木镰刀菌NRRL 13495滤液中，根据培养条件，每升含有高达458±60（平均值±标准误差；n = 3）毫克的4,15 - 二乙酰氧基镰刀菌烯醇。用乙酸乙酯萃取、在硅胶柱上进行色谱分离以及从乙酸乙酯和己烷的混合物中结晶，以96%的产率得到纯物质。
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