Vesonder R F, Ellis J J, Kwolek W F, DeMarini D J
Appl Environ Microbiol. 1982 Apr;43(4):967-70. doi: 10.1128/aem.43.4.967-970.1982.
Two vomitoxin-producing isolates of Fusarium spp. were grown on cracked corn for 1 to 8 weeks at 15, 20, 25, 28, and 32 degrees C. Maximum production of vomitoxin by Fusarium graminearum Schw. NRRL 5883 occurred at 30 degrees C and 40 days, and that by Fusarium roseum Schw. NRRL 6101 occurred at 26 degrees C and 41 days. These optimum production points were determined from response surface contour graphs in relation to temperature and time. Only small amounts of vomitoxin were produced at 15 and 20 degrees C by each strain. A 133-microgram quantity of vomitoxin, with an indicated purity of 95%, was isolated per gram of corn fermented with F. graminearum NRRL 5883.
将两株产呕吐毒素的镰刀菌属分离菌株在破碎玉米上于15、20、25、28和32摄氏度下培养1至8周。禾谷镰刀菌Schw. NRRL 5883产生呕吐毒素的量在30摄氏度和40天时达到最大,粉红镰刀菌Schw. NRRL 6101产生呕吐毒素的量在26摄氏度和41天时达到最大。这些最佳产毒点是根据温度和时间的响应面等高线图确定的。在15和20摄氏度时,每个菌株仅产生少量呕吐毒素。每克用禾谷镰刀菌NRRL 5883发酵的玉米中分离出133微克呕吐毒素,其标示纯度为95%。