利用 PercevalHR 可视化幼虫的细胞质 ATP。

Department of Biology, Duke University, Box 90338, Durham, NC 27708, USA.

Department of Cell Biology, Duke University Medical Center, Durham, NC 27708, USA.

STAR Protoc. 2022 May 26;3(2):101429. doi: 10.1016/j.xpro.2022.101429. eCollection 2022 Jun 17.

Measuring ATP levels within the cytosol of living cells in animals is important to understand how cellular activities are energetically supported, but is challenging because of tissue complexity and ATP sensor limitations. In this protocol, we describe how to quantify ATP levels using PercevalHR in larvae during anchor cell invasion. PercevalHR is a fluorescent biosensor that reports the cytoplasmic ATP:ADP ratio. The protocol can be adapted to analyze the ATP:ADP ratios within other cell types in . For complete details on the use and execution of this protocol, please refer to Garde et al. (2022).

测量动物细胞浆中的 ATP 水平对于了解细胞活动的能量支持方式非常重要，但由于组织复杂性和 ATP 传感器的限制，这是一项具有挑战性的任务。在本方案中，我们描述了如何在幼虫的锚定细胞入侵过程中使用 PercevalHR 来定量检测 ATP 水平。PercevalHR 是一种荧光生物传感器，可报告细胞质中的 ATP:ADP 比值。该方案可以适应于分析中其他细胞类型的 ATP:ADP 比值。有关该方案使用和执行的完整详细信息，请参阅 Garde 等人。（2022 年）。