Institute of Molecular and Cellular Biology, College of Life Science, National Taiwan University, Taipei, Taiwan.
Institute of Molecular Medicine and Center for Precision Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
STAR Protoc. 2021 Mar 18;2(2):100402. doi: 10.1016/j.xpro.2021.100402. eCollection 2021 Jun 18.
Live-cell imaging analysis provides tremendous information for the study of cellular events such as growth cone migration in neuronal development. Here, we describe a protocol for live-cell imaging of migrating PVD dendritic growth cones in the nematode by spinning-disk confocal microscopy. Fluorescently labeled growth cones and cytoskeletal proteins could be continuously observed for 4-6 h in mid-stage larvae. This protocol is suitable for revealing the dynamic molecular and cellular events in dendrite and axon development of . For complete details on the use and execution of this protocol, please refer to Chen et al. (2019).
活细胞成像分析为研究细胞事件提供了大量信息，例如神经元发育中的生长锥迁移。在这里，我们通过旋转盘共聚焦显微镜描述了线虫中 PVD 树突状生长锥迁移的活细胞成像方案。在中期幼虫中，可以连续观察到荧光标记的生长锥和细胞骨架蛋白 4-6 小时。该方案适用于揭示线虫树突和轴突发育中动态的分子和细胞事件。有关该方案使用和执行的完整详细信息，请参见 Chen 等人。(2019 年)。
