Aloraij Yumna, Alsheikh Alanoud, Alyousef Reema A, Alhamlan Fatimah, Suaifan Ghadeer A R Y, Muthana Saddam, Al-Kattan Khaled, Zourob Mohammed
Alfaisal University, Al Zahrawi Street, Al Maather, Al Takhassusi Rd, Riyadh 11533, Saudi Arabia.
King Faisal Specialist Hospital and Research Center, Zahrawi Street, Al Maather, Riyadh 12713, Saudi Arabia.
ACS Omega. 2022 May 19;7(21):17555-17562. doi: 10.1021/acsomega.1c07022. eCollection 2022 May 31.
Despite progress in fighting infectious diseases, human pathogenesis and death caused by infectious diseases remain relatively high worldwide exceeding that of cancer and cardiovascular diseases. Human adenovirus (HAdV) infects cells of the upper respiratory tract causing flu-like symptoms that are accompanied by pain and inflammation. Diagnosis of HAdV is commonly achieved by conventional methods such as viral cultures, immunoassays, and polymerase chain reaction (PCR) techniques. However, there are a variety of problems with conventional methods including slow isolation and propagation, inhibition by neutralizing antibodies, low sensitivity of immunoassays, and the diversity of HAdV strains for the PCR technique. Herein, we report the development and evaluation of a novel, simple, and reliable nanobased immunosensing technique for the rapid detection of human adenoviruses (HAdVs) that cause eye infections. This rapid and low-cost assay can be used for screening and quantitative tests with a detection limit of 10 pfu/mL in less than 2 min. The sensing platform is based on a sandwich assay that can detect HAdVs visually by a color change. Sensor specificity was demonstrated using other common viral antigens, including Flu A, Flu B, coronavirus (COV), and Middle East respiratory syndrome coronavirus (MERS COV). This cotton-based testing device potentially exhibits many of the desired characteristics of a suitable point-of-care and portable test, which can be carried out by nurses or clinicians especially for low-resource settings.
尽管在抗击传染病方面取得了进展，但全球范围内由传染病引起的人类发病和死亡人数仍然相对较高，超过了癌症和心血管疾病。人腺病毒（HAdV）感染上呼吸道细胞，引起类似流感的症状，并伴有疼痛和炎症。HAdV的诊断通常通过传统方法实现，如病毒培养、免疫测定和聚合酶链反应（PCR）技术。然而，传统方法存在各种问题，包括分离和繁殖缓慢、被中和抗体抑制、免疫测定灵敏度低以及PCR技术对HAdV毒株的多样性。在此，我们报告了一种新型、简单且可靠的基于纳米的免疫传感技术的开发和评估，用于快速检测引起眼部感染的人腺病毒（HAdVs）。这种快速且低成本的检测方法可用于筛选和定量测试，在不到2分钟内检测限为10 pfu/mL。传感平台基于夹心测定法，可通过颜色变化直观地检测HAdVs。使用其他常见病毒抗原，包括甲型流感病毒、乙型流感病毒、冠状病毒（COV）和中东呼吸综合征冠状病毒（MERS COV），证明了传感器的特异性。这种基于棉花的检测设备可能具有许多适用于即时护理和便携式检测的理想特性，可由护士或临床医生进行操作，特别是在资源匮乏的环境中。
