Jauregui Alexa M, Liu Yuan, Bhattacharya Sanjoy K, Lee Richard K
Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, 33136, USA.
Miami Integrative Metabolomics Research Center, Miami, FL, 33136, USA.
Data Brief. 2022 May 21;42:108306. doi: 10.1016/j.dib.2022.108306. eCollection 2022 Jun.
This metabolite dataset was collected from transgenic murine retinal ganglion cells (RGC) expressing bacterial channelrhodopsin labeled with fluorescent protein. Mice were subjected to optic nerve crush (ONC) with subsequent RGC stimulation of channelrhodopsin with blue light (promoting regeneration) or non-stimulation (control). ONC induces retinal ganglion cell degeneration over time with progressive loss of axons. In transgenic bacterial channelrhodopsin expressing RGC cells, light stimulation promotes regeneration of ONC axons. Genetically matched wild-type uninjured optic nerves were analyzed as controls for comparison. Metabolites were carefully extracted from finely minced optic nerve tissue using a solvent system (initial separation using 1:1 methanol and HO and second extraction using 8:1:1 of acetonitrile:acetone:methanol). Untargeted liquid chromatography-mass spectrometry profiling was performed using fractionation on a Vanquish Horizon Binary UHPLC. Subsequent analyses were performed on an inline coupled Q-Exactive Orbitrap instrument. Metabolites were identified using Compound Discoverer software. Statistical analysis was performed using MetaboAnalyst 5.0. This data is available on Metabolomics Workbench, Study ID ST002111.
该代谢物数据集是从表达带有荧光蛋白标记的细菌视紫红质的转基因小鼠视网膜神经节细胞（RGC）中收集的。对小鼠进行视神经挤压（ONC），随后用蓝光刺激（促进再生）或不刺激（对照）RGC的视紫红质。随着时间的推移，ONC会导致视网膜神经节细胞变性，并伴有轴突的逐渐丧失。在表达细菌视紫红质的转基因RGC细胞中，光刺激可促进ONC轴突的再生。将基因匹配的野生型未受伤视神经作为对照进行分析以作比较。使用溶剂系统（先用1:1甲醇和水进行初步分离，再用8:1:1的乙腈：丙酮：甲醇进行二次提取）从精细切碎的视神经组织中仔细提取代谢物。使用Vanquish Horizon二元超高效液相色谱仪进行分馏，进行非靶向液相色谱-质谱分析。随后在在线联用的Q-Exactive Orbitrap仪器上进行分析。使用Compound Discoverer软件鉴定代谢物。使用MetaboAnalyst 5.0进行统计分析。该数据可在代谢组学工作台（Study ID ST002111）上获取。
