Arcuri Jennifer, Veldman Matthew B, Bhattacharya Sanjoy K
Bascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, 33136, USA.
Miami Integrative Metabolomics Research Center, Miami, FL, 33136, USA.
Data Brief. 2021 Jul 14;37:107260. doi: 10.1016/j.dib.2021.107260. eCollection 2021 Aug.
The right optic nerve of adult, 6 month to 1 year old, female and male were crushed and collected three days after. Matching controls of uninjured left optic nerves were also collected. The tissue was dissected from euthanized fish and frozen on dry ice. Samples were pooled for each category (female crush, female control, male crush, male control) n = 24 to obtain sufficient tissue for analysis. The brain from one male fish was also collected for control/calibration. Lipid extraction was done with the Bligh and Dyer [1] method, followed by untargeted liquid chromatography-mass spectrometry (LC MS-MS) lipid profiling using a Q-Exactive Orbitrap instrument coupled with Vanquish Horizon Binary UHPLC LC-MS system. The lipids were identified and quantified with LipidSearch 4.2.21 and the statistical analysis was conducted through Metaboanalyst 5.0. This data is available at Metabolomics Workbench, Study ID ST001725.
对成年（6个月至1岁）雌鱼和雄鱼的右侧视神经进行挤压，并在三天后收集样本。同时收集未受伤的左侧视神经作为匹配对照。组织取自安乐死的鱼，并在干冰上冷冻。将每个类别（雌性挤压组、雌性对照组、雄性挤压组、雄性对照组）的样本合并，每组n = 24，以获得足够的组织用于分析。还收集了一条雄鱼的大脑作为对照/校准样本。采用布莱和戴尔[1]法进行脂质提取，随后使用配备Vanquish Horizon二元超高效液相色谱-质谱系统的Q-Exactive Orbitrap仪器进行非靶向液相色谱-质谱联用（LC MS-MS）脂质谱分析。使用LipidSearch 4.2.21对脂质进行鉴定和定量，并通过Metaboanalyst 5.0进行统计分析。该数据可在代谢组学工作台获取，研究ID为ST001725。
