Applied Virology Research Center, Baqiyatallah University of Medical sciences, Tehran, Iran.
Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Biotechnol Bioeng. 2022 Sep;119(9):2305-2312. doi: 10.1002/bit.28149. Epub 2022 Jun 16.
Human papillomavirus (HPV) is a causative agent of cervical cancer among women worldwide. Serological and molecular tests are commonly used to detect and identify HPV, but all the detection methods for HPV have some limitations. Nowadays, considerable advancements in nanosensors have enabled monitoring of hybridization procedures dynamically for HPV detection. Biosensors, as effective, quick, economical, and highly sensitive tools, can be used in the diagnosis of HPV as an alternative technique instead of other detection methods. Biosensor detection methods of HPV in use from 2000 to 2021 were investigated using several databases such as Google Scholar, Scopus, PubMed, and the Scientific Information Database. Furthermore, a manual search of the references of the retrieved articles was performed. On analyzing the most recently developed biosensors for HPV identification, we observed that three biosensor systems, electrochemical, optical, and piezoelectric systems, are the main transducers used in the development of HPV biosensors. The aim of this review is to examine recent research on biosensors for the detection of HPV and perform a comparison with other diagnostic methods. Considering the importance of rapid HPV detection in the control of infection and development of public health measures, improvement of biosensors as an economical and quick method can be very useful in the diagnosis of HPV.
人乳头瘤病毒（HPV）是全球女性宫颈癌的致病因子。血清学和分子检测通常用于检测和识别 HPV，但 HPV 的所有检测方法都存在一些局限性。如今，纳米传感器的巨大进步使得 HPV 检测的杂交过程可以动态监测。生物传感器作为一种有效、快速、经济和高灵敏度的工具，可以作为替代其他检测方法的技术，用于 HPV 的诊断。利用 Google Scholar、Scopus、PubMed 和科学信息数据库等多个数据库，对 2000 年至 2021 年期间使用的 HPV 生物传感器检测方法进行了研究。此外，还对检索到的文章的参考文献进行了手动搜索。在分析最近开发的 HPV 识别生物传感器时，我们观察到电化学、光学和压电系统这三种生物传感器系统是 HPV 生物传感器开发中主要的传感器。本综述旨在考察用于 HPV 检测的生物传感器的最新研究，并与其他诊断方法进行比较。考虑到快速检测 HPV 在控制感染和制定公共卫生措施方面的重要性，作为一种经济、快速的方法，生物传感器的改进在 HPV 诊断方面非常有用。
