Division of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido, 001-0020, Japan.
Division of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido, 001-0020, Japan; Shionogi & Co., Ltd., Osaka, 541-0045, Japan.
Biochem Biophys Res Commun. 2022 Aug 6;616:115-121. doi: 10.1016/j.bbrc.2022.05.080. Epub 2022 May 27.
The genus Flavivirus includes pathogenic tick- and mosquito-borne flaviviruses as well as non-pathogenic insect-specific flaviviruses (ISFVs). Phylogenetic analysis based on whole amino acid sequences has indicated that lineage II ISFVs have similarities to pathogenic flaviviruses. In this study, we used reactive analysis with immune serum against Psorophora flavivirus (PSFV) as a lineage IIa ISFV, and Barkeji virus (BJV) as a lineage IIb ISFV, to evaluate the antigenic similarity among lineage IIa and IIb ISFVs, and pathogenic mosquito-borne flaviviruses (MBFVs). Binding and antibody-dependent enhancement assays showed that anti-PSFV sera had broad cross-reactivity with MBFV antigens, while anti-BJV sera had low cross-reactivity. Both of the lineage II ISFV antisera were rarely observed to neutralize MBFVs. These results suggest that lineage IIa ISFV PSFV has more antigenic similarity to MBFVs than lineage IIb ISFV BJV.
黄病毒属包括致病的蜱传和蚊传黄病毒以及非致病的昆虫特异性黄病毒（ISFV）。基于全氨基酸序列的系统发育分析表明，谱系 II ISFV 与致病性黄病毒具有相似性。在这项研究中，我们使用针对 Psorophora flavivirus（PSFV）的免疫血清进行反应性分析，将其作为谱系 IIa ISFV，并用 Barkeji 病毒（BJV）作为谱系 IIb ISFV，评估谱系 IIa 和 IIb ISFV 与致病性蚊媒黄病毒（MBFV）之间的抗原相似性。结合和抗体依赖性增强测定表明，抗 PSFV 血清与 MBFV 抗原具有广泛的交叉反应性，而抗 BJV 血清的交叉反应性较低。两种谱系 II ISFV 抗血清均很少观察到中和 MBFV 的作用。这些结果表明，谱系 IIa ISFV PSFV 与 MBFV 的抗原相似性高于谱系 IIb ISFV BJV。
