Department of Cardiology, Wuhan Asia General Hospital, Wuhan, China.
Department of Critical Care Medicine, Wuhan Asia General Hospital, Wuhan, China.
J Clin Lab Anal. 2022 Jul;36(7):e24546. doi: 10.1002/jcla.24546. Epub 2022 Jun 6.
Brain-derived neurotrophic factor (BDNF) regulates the lipid metabolism, atherosclerosis plaque formation, and inflammatory process, while the study about its clinical role in coronary heart disease (CHD) is few. The present study intended to explore the expression of BDNF and its relationship with stenosis, inflammation, and adhesion molecules in CHD patients.
After serum samples were obtained from 207 CHD patients, BDNF, tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β, IL-6, IL-8, IL-17A, vascular cell adhesion molecule-1 (VCAM-1), and intercellular adhesion molecule-1 (ICAM-1) levels were determined using ELISA. Then, the BDNF level was also examined in 40 disease controls (DCs) and 40 healthy controls (HCs), separately.
BDNF was lower in CHD patients than in DCs and HCs (median (95% confidential interval) value: 5.6 (3.5-9.6) ng/mL vs. 10.7 (6.1-17.0) ng/mL and 12.6 (9.4-18.2) ng/mL, both p < 0.001). BDNF could well distinguish CHD patients from DCs (area under the curve [AUC]: 0.739) and HCs (AUC: 0.857). BDNF was negatively associated with triglyceride (p = 0.014), total cholesterol (p = 0.037), and low-density lipoprotein cholesterol (p = 0.008). BDNF was negatively associated with CRP (p < 0.001), TNF-α (p < 0.001), IL-1β (p = 0.008), and IL-8 (p < 0.001). BDNF was negatively related to VCAM-1 (p < 0.001) and ICAM-1 (p = 0.003). BDNF was negatively linked with the Gensini score (p < 0.001).
BDNF reflects the lipid dysregulation, inflammatory status, and stenosis degree in CHD patients.
脑源性神经营养因子（BDNF）可调节脂代谢、动脉粥样硬化斑块形成和炎症过程，而有关其在冠心病（CHD）中的临床作用的研究较少。本研究旨在探讨 BDNF 的表达及其与 CHD 患者狭窄、炎症和黏附分子的关系。
从 207 例 CHD 患者中抽取血清样本后，采用 ELISA 法测定 BDNF、肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-1β、IL-6、IL-8、IL-17A、血管细胞黏附分子-1（VCAM-1）和细胞间黏附分子-1（ICAM-1）水平。然后，分别检测 40 例疾病对照（DCs）和 40 例健康对照（HCs）中的 BDNF 水平。
CHD 患者的 BDNF 水平低于 DCs 和 HCs（中位数（95%可信区间）值：5.6（3.5-9.6）ng/ml 比 10.7（6.1-17.0）ng/ml 和 12.6（9.4-18.2）ng/ml，均 P<0.001）。BDNF 可很好地区分 CHD 患者与 DCs（曲线下面积[AUC]：0.739）和 HCs（AUC：0.857）。BDNF 与甘油三酯（p=0.014）、总胆固醇（p=0.037）和低密度脂蛋白胆固醇（p=0.008）呈负相关。BDNF 与 CRP（p<0.001）、TNF-α（p<0.001）、IL-1β（p=0.008）和 IL-8（p<0.001）呈负相关。BDNF 与 VCAM-1（p<0.001）和 ICAM-1（p=0.003）呈负相关。BDNF 与 Gensini 评分呈负相关（p<0.001）。
BDNF 反映了 CHD 患者的脂代谢紊乱、炎症状态和狭窄程度。
