Department of Oriental Medicine Resources and Institute of Korean Medicine Industry, Mokpo National University, Mokpo, Republic of Korea.
Department of Food and Nutrition, Mokpo National University, Mokpo, Republic of Korea.
Phytother Res. 2019 Oct;33(10):2765-2774. doi: 10.1002/ptr.6462. Epub 2019 Aug 5.
The purpose of this study was to evaluate the pancreatic beta cell protective and glucose uptake enhancing effect of the water extract of Tinospora cordifolia stem (TCSE) by using rat insulinoma (RIN)-m5F cells and 3 T3-L1 adipocytes. RIN-m5F cells were stimulated with interleukin-1β and interferon-γ, and the effect of TCSE on insulin secretion and cytokine-induced toxicity was measured by ELISA and MTT assay, respectively. The glucose uptake and protein expression were measured by fluorometry and western blotting. Antidiabetic effect of TCSE was measured using streptozotocin-induced diabetic rats. TCSE dose dependently increased cell viability and insulin secretion in RIN-m5F cells. In addition, TCSE increased both the glucose uptake and glucose transporter 4 translocation in 3 T3-L1 adipocytes via PI3K pathway. Finally, TCSE significantly lowered blood glucose and diet intake and increased body weight in streptozotocin-induced diabetic rats. The level of serum insulin and hepatic glycogen was increased, whereas the level of serum triglyceride, total cholesterol, dipeptidyl peptidase-4, and thiobarbituric acid reactive substances was decreased in TCSE-administered rats. TCSE also increased glucose transporter 4 protein expression in the adipose tissue and liver of TCSE-fed diabetic rats. Our results suggested that TCSE preserved RIN-m5F cells from cytokine-induced toxicity and enhanced glucose uptake in 3 T3-L1 adipocytes, which may regulate glucose metabolism in diabetic rats.
本研究旨在通过使用大鼠胰岛素瘤（RIN）-m5F 细胞和 3T3-L1 脂肪细胞来评估三叶鬼针草茎水提取物（TCSE）对胰腺β细胞的保护作用和增强葡萄糖摄取的作用。用白细胞介素-1β和干扰素-γ刺激 RIN-m5F 细胞，通过 ELISA 和 MTT 测定分别测定 TCSE 对胰岛素分泌和细胞因子诱导的毒性的影响。通过荧光计和 Western blot 测定葡萄糖摄取和蛋白表达。用链脲佐菌素诱导的糖尿病大鼠测定 TCSE 的抗糖尿病作用。TCSE 呈剂量依赖性地增加 RIN-m5F 细胞中的细胞活力和胰岛素分泌。此外，TCSE 通过 PI3K 途径增加了 3T3-L1 脂肪细胞中的葡萄糖摄取和葡萄糖转运蛋白 4 易位。最后，TCSE 显著降低了链脲佐菌素诱导的糖尿病大鼠的血糖和饮食摄入量，并增加了体重。血清胰岛素和肝糖原水平升高，而血清甘油三酯、总胆固醇、二肽基肽酶-4 和硫代巴比妥酸反应物质水平降低在 TCSE 给药大鼠中。TCSE 还增加了 TCSE 喂养的糖尿病大鼠脂肪组织和肝脏中的葡萄糖转运蛋白 4 蛋白表达。我们的结果表明，TCSE 可防止 RIN-m5F 细胞免受细胞因子诱导的毒性，并增强 3T3-L1 脂肪细胞中的葡萄糖摄取，这可能调节糖尿病大鼠的葡萄糖代谢。
