Dekomah Simon Dontoro, Wang Yihao, Qin Tianyuan, Xu Derong, Sun Chao, Yao Panfeng, Liu Yuhui, Bi Zhenzhen, Bai Jiangping
College of Agronomy, Gansu Agricultural University, Lanzhou, China.
Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou, China.
Front Genet. 2022 May 24;13:874397. doi: 10.3389/fgene.2022.874397. eCollection 2022.
Calcium-dependent protein kinases (CDPKs) are a class of serine/threonine protein kinases encoded by several gene families that play key roles in stress response and plant growth and development. In this study, the BLAST method was used to search for protein sequences of the potato Calcium-dependent protein kinase gene family. The chromosome location, phylogeny, gene structures, gene duplication, cis-acting elements, protein-protein interaction, and expression profiles were analyzed. Twenty-five CDPK genes in the potato genome were identified based on RNA-seq data and were clustered into four groups (I-IV) based on their structural features and phylogenetic analysis. The result showed the composition of the promoter region of the gene, including light-responsive elements such as Box4, hormone-responsive elements such as ABRE, and stress-responsive elements such as MBS. Four pairs of segmental duplications were found in StCDPKs genes and the Ka/Ks ratios were below 1, indicating a purifying selection of the genes. The protein-protein interaction network revealed defense-related proteins such as; respiratory burst oxidase homologs (RBOHs) interacting with potato CDPKs. Transcript abundance was measured RT-PCR between the two cultivars and their relative expression of CDPK genes was analyzed after 15, 20, and 25 days of drought. There were varied expression patterns of and , between the two potato cultivars under mannitol induced-drought conditions. Correlation analysis showed that and may be the major differentially expressed genes involved in the regulation of malondialdehyde (MDA) and proline content in response to drought stress, opening a new research direction for genetic improvement of drought resistance in potato.
钙依赖蛋白激酶（CDPKs）是一类由多个基因家族编码的丝氨酸/苏氨酸蛋白激酶，在应激反应以及植物生长发育过程中发挥关键作用。在本研究中，采用BLAST方法搜索马铃薯钙依赖蛋白激酶基因家族的蛋白质序列。分析了其染色体定位、系统发育、基因结构、基因复制、顺式作用元件、蛋白质-蛋白质相互作用以及表达谱。基于RNA测序数据在马铃薯基因组中鉴定出25个CDPK基因，并根据其结构特征和系统发育分析将它们聚类为四组（I-IV）。结果显示了该基因启动子区域的组成，包括如Box4等光响应元件、如ABRE等激素响应元件以及如MBS等应激响应元件。在StCDPKs基因中发现了四对片段重复，且Ka/Ks比值低于1，表明这些基因受到纯化选择。蛋白质-蛋白质相互作用网络揭示了与防御相关的蛋白质，如与马铃薯CDPKs相互作用的呼吸爆发氧化酶同源物（RBOHs）。通过RT-PCR测定了两个品种之间的转录本丰度，并在干旱处理15、20和25天后分析了CDPK基因的相对表达。在甘露醇诱导的干旱条件下，两个马铃薯品种之间的 和 具有不同的表达模式。相关性分析表明， 和 可能是参与调控丙二醛（MDA）和脯氨酸含量以响应干旱胁迫的主要差异表达基因，为马铃薯抗旱性的遗传改良开辟了新的研究方向。
