Genome-Wide Identification and Characterization of () and () Gene Families in .

Calcium-dependent protein kinase (CDPK or CPK) and CDPK-related kinase (CRK) play an important role in plant growth, development, and adaptation to environmental stresses. However, their gene families had been yet inadequately investigated in . In this study, six genes were computationally identified, they were classified into five groups with based on phylogenetic relationships. Six pairs of segmental duplications were observed in and genes and the ratio, an indicator of selection pressure, was below 0.310, indicating that these gene pairs underwent strong purifying selection. -acting elements of morphogenesis, multiple hormone responses, and abiotic stresses were predicted in the promoter region. The spatial expression of and displays diversity. The expression of and could be regulated by various stresses. MtCDPK4, 14, 16, 22, and MtCRK6 harbor both N-myristoylation site and palmitoylation site and were anchored on plasma membrane, while MtCDPK7, 9, and 15 contain no or only one N-acylation site and were distributed in cytosol and nucleus, suggesting that the N-terminal acylation sites play a key role in subcellular localization of MtCDPKs and MtCRKs. In summary, comprehensive characterization of and provide a subset of candidate genes for further functional analysis and genetic improvement against drought, cold, salt and biotic stress.