Taniguchi K, Shoji H, Solomonow M, Yazdani M, Nakamoto T
Biochem Med Metab Biol. 1987 Feb;37(1):125-31. doi: 10.1016/0885-4505(87)90018-1.
We studied the biochemical effects of excess methionine intake on the skin of newborn rats. Group 1 pups were intubated with methionine dissolved in 0.1 ml physiological saline solution in the amount of 0.1 g/100 body wt as a control using a gastric needle. Group 2 pups were given 0.2 g/100 g in the same manner as group 1 as an experimental group. They were intubated every other day from day 3 to 13. On Day 15, [14C]proline was injected intraperitoneally into pups and their skin was removed. 14C total and hydroxyproline uptake was examined in the tissue as well as in the sequential extracts. Although excess methionine intake by the pups did not alter the collagen content of their skin, it caused an increase in the content of type III collagen and a decrease in crosslinked collagen. In addition, newly synthesized collagen in the neutral salt extract increased in the excess methionine group, indicating that crosslinked collagen decreases as excess methionine was intubated. The present study demonstrated that excess methionine in the early lactational period altered the nature of the skin collagen of suckling newborn rat pups.
我们研究了过量摄入蛋氨酸对新生大鼠皮肤的生化影响。第1组幼崽用胃针经口插管给予溶解于0.1 ml生理盐水中的蛋氨酸，剂量为0.1 g/100体重，作为对照组。第2组幼崽以与第1组相同的方式给予0.2 g/100 g蛋氨酸，作为实验组。从第3天到第13天，每隔一天对它们进行插管。在第15天，将[14C]脯氨酸腹腔注射到幼崽体内，然后取下它们的皮肤。检测了组织以及连续提取物中的14C总量和羟脯氨酸摄取量。虽然幼崽过量摄入蛋氨酸并未改变其皮肤中的胶原蛋白含量，但导致III型胶原蛋白含量增加，交联胶原蛋白含量减少。此外，过量蛋氨酸组中性盐提取物中新合成的胶原蛋白增加，表明随着经口给予过量蛋氨酸，交联胶原蛋白减少。本研究表明，哺乳期早期过量的蛋氨酸改变了哺乳新生大鼠幼崽皮肤胶原蛋白的性质。
