Programa de Pós-Graduação em Biologia Experimental, Universidade Federal de Rondônia, Porto Velho, RO, Brazil; Laboratório de Entomologia, Fundação Oswaldo Cruz, Fiocruz Rondônia, Porto Velho, RO, Brazil.
Laboratório de Entomologia, Fundação Oswaldo Cruz, Fiocruz Rondônia, Porto Velho, RO, Brazil; Instituto Nacional de Epidemiologia da Amazônia Ocidental (INCT-EpiAMO), Porto Velho, RO, Brazil.
Acta Trop. 2022 Sep;233:106550. doi: 10.1016/j.actatropica.2022.106550. Epub 2022 Jun 6.
One strategy to identify transmission foci is based on vector monitoring, and efficient methods are important for vector control. Our study evaluated different light sources (red, green, ultraviolet, blue, and incandescent light) to capture sand fly in Porto Velho, Brazil. We also evaluated Leishmania and blood meal sources in females. A total of 1,943 individuals were identified in 45 species level-taxa, with Trichophoromyia ubiquitalis (Mangabeira) (n = 364), Nyssomyia antunesi (n = 241), Bichromomyia flaviscutellata (Mangabeira) (n = 222), and Psychodopygus davisi (Root) (n = 148) being the most abundant. Incandescent light captured most individuals (n = 589), followed by blue (n = 471), green (n = 452), ultraviolet (n = 281) and red (n = 150). No significant difference was observed between the species composition and lights (PERMANOVA: Pseudo F = 1.29, p = 0.14, NMDS: Stress 0.18). The Shannon and Simpson indices demonstrated a high diversity captured using all lights. Our data demonstrated that LEDs are alternative devices for sand fly capture, with blue and green LEDs presenting similar results to incandescent light. 53 pools were analyzed, only one pool was positive for kDNA and hsp70 targets [Nyssomyia fraihai (Martins, Falão & Silva)] and identified as Endotrypanum spp., suggesting that other trypanosomatids may circulate in the locality. Choloepus hoffmanni (two-toed sloth) Peters, Homo sapiens Linnaeus, Proechimys gardneri (Gardner's spiny rat) Da Silva, and Tamandua tetradactyla (lesser anteater) (Linnaeus) were blood meal sources identified in females, increasing the knowledge about sources used by these insects.
一种识别传播焦点的策略是基于媒介监测，而有效的方法对于媒介控制很重要。我们的研究评估了不同的光源（红色、绿色、紫外线、蓝色和白炽灯）在巴西波多韦柳捕获沙蝇的效果。我们还评估了雌虫中的莱什曼原虫和血食源。在 45 个种级分类单元中鉴定出 1943 个人，其中 Trichophoromyia ubiquitalis (Mangabeira)（n=364）、Nyssomyia antunesi（n=241）、Bichromomyia flaviscutellata (Mangabeira)（n=222）和 Psychodopygus davisi (Root)（n=148）最为丰富。白炽灯捕获的个体最多（n=589），其次是蓝色（n=471）、绿色（n=452）、紫外线（n=281）和红色（n=150）。物种组成和灯光之间没有观察到显著差异（PERMANOVA：伪 F=1.29，p=0.14，NMDS：Stress 0.18）。香农和辛普森指数表明，所有灯光都能捕获到高度多样化的沙蝇。我们的数据表明，LED 是捕获沙蝇的替代设备，蓝色和绿色 LED 与白炽灯具有相似的效果。分析了 53 个池，只有一个池的 kDNA 和 hsp70 靶标（Nyssomyia fraihai (Martins, Falão & Silva)）呈阳性，鉴定为 Endotrypanum spp.，表明其他锥虫可能在该地区循环。鉴定出雌性的血食源包括 Choloepus hoffmanni（二趾树懒）、Homo sapiens Linnaeus、Proechimys gardneri（ Gardner 的刺鼠）和 Tamandua tetradactyla（小食蚁兽）（Linnaeus），增加了对这些昆虫使用的来源的了解。
