Camellix Research Laboratory, Augusta, GA, USA.
Department of Oral Biology & Diagnostic Sciences, Dental College of Georgia, Augusta University, Augusta, GA, USA.
Am J Infect Control. 2022 Nov;50(11):1212-1219. doi: 10.1016/j.ajic.2022.05.027. Epub 2022 Jun 4.
Non-toxic hand hygiene and surface disinfectant products with virucidal activity against alcohol-resistant nonenveloped norovirus are in urgent need.
Alcohol-based formulations were made with epigallocatechin-3-gallate-palmitate (EC16), an FDA accepted food additive. Based on in-house testing of formulations, 3 prototypes, PTV80 hand gel, PST70 surface disinfectant spray and PST70 surface disinfectant wipe, were selected from in-house tests for independent testing at GLP (good laboratory practice) laboratories according to EN 14476:2019 (hand gel), ASTM test method E1053-20 (spray), and ASTM E2362-15, E1053, and ASTM E2896-12 (wipe).
The PTV80 hand gel prototype demonstrated a >99.999% reduction of murine norovirus S99 infectivity in 60 seconds. Carrier testing of the PST70 surface spray and surface wipe demonstrated reduction of feline calicivirus infectivity by >99.99% in 60 seconds. In addition, testing with human coronavirus and human herpes simplex virus demonstrated >99.99% efficacy in 60 seconds, consistent with broad spectrum virucidal activity.
The novel non-toxic prototypes containing EC16 were found to be suitable for use in future hand sanitizer gel, surface disinfectant spray and wipe products against norovirus. Products based on these formulations could be used safely to help prevent and control norovirus and other emerging virus outbreaks, pending future studies.
急需具有抗酒精包膜诺如病毒病毒活性的无毒手部卫生和表面消毒剂产品。
使用表没食子儿茶素没食子酸酯-十六酸酯（EC16）制成基于酒精的配方，EC16 是一种获得 FDA 认可的食品添加剂。根据配方的内部测试，从内部测试中选择了 3 种原型产品，PTV80 手部凝胶、PST70 表面消毒剂喷雾和 PST70 表面消毒剂擦拭巾，根据 EN 14476：2019（手部凝胶）、ASTM 测试方法 E1053-20（喷雾）和 ASTM E2362-15、E1053 和 ASTM E2896-12（擦拭巾），在 GLP（良好实验室规范）实验室进行独立测试。
PTV80 手部凝胶原型在 60 秒内可使鼠诺如病毒 S99 的感染性降低 99.999%以上。PST70 表面喷雾和表面擦拭的载体测试表明，在 60 秒内可使猫杯状病毒的感染性降低 99.99%以上。此外，用人类冠状病毒和单纯疱疹病毒进行的测试表明，在 60 秒内的有效率>99.99%，具有广谱杀病毒活性。
含有 EC16 的新型无毒原型被发现适用于未来的手部卫生凝胶、表面消毒剂喷雾和擦拭产品，以对抗诺如病毒。基于这些配方的产品可安全用于帮助预防和控制诺如病毒和其他新兴病毒的爆发，等待未来的研究。
