Tudela J, García Cánovas F, Varón R, García Carmona F, Gálvez J, Lozano J A
Biochim Biophys Acta. 1987 Apr 30;912(3):408-16. doi: 10.1016/0167-4838(87)90046-x.
This paper deals with the kinetic study of reaction mechanisms with enzyme inactivation induced by a suicide substrate in the presence or absence of an auxiliary substrate and in conditions of excess of substrates in relation to the enzyme concentration and vice versa. A transient-phase approach has been developed that enables explicit equations with one or two significant exponentials to be obtained, thereby showing the dependence of product concentration on time. The validity of these equations has been checked, and a comparison made with those previously obtained by other authors. We propose an experimental design to determine the corresponding parameters and kinetic constants. The simplicity of our method allows a systematic application to more complex mechanisms.
本文研究了在有或没有辅助底物存在的情况下，以及在底物相对于酶浓度过量或反之的条件下，自杀底物诱导酶失活的反应机制的动力学。已开发出一种瞬态相方法，该方法能够获得具有一个或两个显著指数的显式方程，从而显示产物浓度对时间的依赖性。已检验了这些方程的有效性，并与其他作者先前获得的方程进行了比较。我们提出了一种实验设计来确定相应的参数和动力学常数。我们方法的简单性允许将其系统地应用于更复杂的机制。
