海湾战争慢性多症状疾病中宿主肠道抗药基因与持续性炎症相关。

Host gut resistome in Gulf War chronic multisymptom illness correlates with persistent inflammation.

机构信息

Environmental Health and Disease Laboratory, Department of Environmental Health Sciences, Arnold School of Public Health, University of South Carolina, Columbia, SC, USA.

Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC, USA.

出版信息

Commun Biol. 2022 Jun 7;5(1):552. doi: 10.1038/s42003-022-03494-7.

DOI:10.1038/s42003-022-03494-7

PMID:35672382

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9174162/

Abstract

Chronic multisymptom illness (CMI) affects a subsection of elderly and war Veterans and is associated with systemic inflammation. Here, using a mouse model of CMI and a group of Gulf War (GW) Veterans' with CMI we show the presence of an altered host resistome. Results show that antibiotic resistance genes (ARGs) are significantly altered in the CMI group in both mice and GW Veterans when compared to control. Fecal samples from GW Veterans with persistent CMI show a significant increase of resistance to a wide class of antibiotics and exhibited an array of mobile genetic elements (MGEs) distinct from normal healthy controls. The altered resistome and gene signature is correlated with mouse serum IL-6 levels. Altered resistome in mice also is correlated strongly with intestinal inflammation, decreased synaptic plasticity, reversible with fecal microbiota transplant (FMT). The results reported might help in understanding the risks to treating hospital acquired infections in this population.

摘要

慢性多症状疾病（CMI）影响一部分老年人群和退伍军人，并与全身炎症有关。在这里，我们使用 CMI 的小鼠模型和一组患有 CMI 的海湾战争（GW）退伍军人，展示了宿主耐药组的改变。结果表明，与对照组相比，CMI 组的小鼠和 GW 退伍军人中的抗生素耐药基因（ARGs）显著改变。患有持续性 CMI 的 GW 退伍军人的粪便样本显示出对广泛类别的抗生素的耐药性显著增加，并表现出与正常健康对照不同的一系列移动遗传元件（MGEs）。改变的耐药组和基因特征与小鼠血清 IL-6 水平相关。在小鼠中改变的耐药组也与肠道炎症强烈相关，突触可塑性降低，粪便微生物群移植（FMT）可逆转。报告的结果可能有助于了解在该人群中治疗医院获得性感染的风险。