Department of Thoracic Surgery, Shanghai Key Laboratory of Tissue Engineering, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.
School of Materials and Chemistry, University of Shanghai for Science and Technology, Shanghai, 200093, People's Republic of China.
J Nanobiotechnology. 2022 Jun 7;20(1):259. doi: 10.1186/s12951-022-01468-9.
Patients with diabetic foot ulcers usually suffer from inefficient epithelisation and angiogenesis accompanied by chronic wound healing. Diabetic foot ulcers remain a major challenge in clinical medicine; however, traditional treatments are incapable of transdermal drug delivery, resulting in a low drug delivery rate. We report the development of TiC MXenes-integrated poly-γ-glutamic acid (γ-PGA) hydrogel microneedles to release asiaticoside (MN-MXenes-AS). Asiaticoside was loaded into PGA-MXenes hydrogel to facilitate cell proliferation while regulating angiogenesis. The characterisation and mechanical strength of the microneedles were investigated in vitro, and the wound-healing efficacy of the microneedles was confirmed in diabetic mice. MXenes significantly improved the mechanical strength of microneedles, while γ-PGA hydrogels provided a moist microenvironment for wound healing. Mice treated with MN-MXenes-AS demonstrated obvious improvements in wound healing process. We successfully fabricated an MXenes-integrated microneedle that possesses sufficient rigidity to penetrate the cuticle for subcutaneous drug delivery, thereby accelerating diabetic wound healing. We demonstrated that MN-MXenes-AS is effective in promoting growth both in vivo and in vitro. Collectively, our data show that MN-MXenes-AS accelerated the healing of diabetic foot ulcers, supporting the use of these microneedles in the treatment of chronic wounds.
糖尿病足溃疡患者通常表现出低效的上皮化和血管生成，并伴有慢性伤口愈合。糖尿病足溃疡仍然是临床医学的一大挑战；然而，传统的治疗方法无法进行透皮药物输送，导致药物输送率低。我们报告了一种 TiC MXenes 整合聚-γ-谷氨酸（γ-PGA）水凝胶微针来释放积雪草苷（MN-MXenes-AS）。将积雪草苷载入 PGA-MXenes 水凝胶中，以促进细胞增殖并调节血管生成。我们对微针的特性和机械强度进行了体外研究，并在糖尿病小鼠中证实了微针的伤口愈合功效。MXenes 显著提高了微针的机械强度，而 γ-PGA 水凝胶为伤口愈合提供了湿润的微环境。经 MN-MXenes-AS 处理的小鼠的伤口愈合过程明显得到改善。我们成功制备了一种 MXenes 整合的微针，它具有足够的刚性穿透角质层进行皮下药物输送，从而加速糖尿病伤口愈合。我们证明了 MN-MXenes-AS 能够有效促进体内和体外的生长。总之，我们的数据表明 MN-MXenes-AS 加速了糖尿病足溃疡的愈合，支持将这些微针用于治疗慢性伤口。
