Wang Bo, Luo Guang-Heng, Wang Zhen, Yang Bing, Xu Yuan-Gao, Sun Zhao-Lin, Tian Ye
Department of Urologic Surgery, Guizhou Provincial People's Hospital, Guiyang, Guizhou, 550002 China.
Graduate School of Guizhou Medical University, Guiyang, Guizhou, 550001 China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2021 May;52(3):477-484. doi: 10.12182/20210560305.
To establish an experimental prostatitis animal model in Sprague-Dawley (SD) rats through induction by treatment of estrogen and androgen at different concentrations.
Fifty-three male SD rats aged 3 to 4 months were used in the study, and the castration model of male rats was established by excision of bilateral testes. The rats were randomly assigned to a blank group, a castration group and treatment groups receiving estrogen and androgen at different concentrations after castration, with 4 rats in each group. Dihydrotestosterone (DHT) and estradiol (E) were administered daily by subcutaneous injection to the treatment groups. All the rats were sacrificed by way of cervical dislocation after 1 month and the serum DHT and E concentrations of the rats in each group were assessed with ELISA. Prostate specimens were collected and the relative weight of the prostate of each group of rats was calculated. After HE staining of the prostate tissue, we observed with optic microscope structural changes in the prostate tissue and the state of prostatic inflammation in each group. Immunohistochemical examination was done to assess the expression of three inflammatory factors, transforming growth factor-β1 (TGF-β1), interleukin (IL)-6 and IL-8, in rat prostate tissues.
The results of HE staining of rat prostate tissue showed that, compared with the blank group and castration group, the degree of inflammation increased significantly in the E0.05+DHT 0.5 mg/kg group and DHT0.15+E0.15 mg/kg group ( <0.05). However, once the concentration of DHT exceeded 0.5 mg/kg, the degree of inflammation did not further aggravate. The results of immunohistochemical staining showed that when the concentration of exogenous E was constant, the expression of TGF-β1 and IL-8 increased significantly in the E0.05+DHT 0.15 mg/kg group, E0.05+DHT 0.5 mg/kg group and E0.05+DHT 1.5 mg/kg group compared with that of the blank group ( <0.05). In the E0.05+DHT 0.15 mg/kg group and E0.05+DHT 0.5 mg/kg group, the expression of TGF-β1 and IL-8 increased significantly compared with that of the castration group ( <0.05). Once the concentration of DHT reached 0.5 mg/kg, further increase in the concentration of DHT did not lead to any significant changes in the expression of TGF-β1 or IL-8. In addition, when the concentration of exogenous DHT remained unchanged, the expressions of TGF-β1, IL-6, and IL-8 increased significantly in the DHT0.15+E 0.05 mg/kg group and DHT0.15+E 0.5 mg/kg group, compared with that of the blank group and castration group ( <0.05).
Castration combined with treatment of different concentrations of estrogen and androgen could successfully induce the prostatitis model in SD rats.
通过不同浓度雌激素和雄激素处理诱导，建立Sprague-Dawley（SD）大鼠实验性前列腺炎动物模型。
选取53只3至4月龄雄性SD大鼠用于本研究，通过切除双侧睾丸建立雄性大鼠去势模型。将大鼠随机分为空白组、去势组以及去势后接受不同浓度雌激素和雄激素处理的治疗组，每组4只。治疗组每日皮下注射二氢睾酮（DHT）和雌二醇（E）。1个月后所有大鼠经颈椎脱臼处死，采用酶联免疫吸附测定法（ELISA）评估每组大鼠血清DHT和E浓度。采集前列腺标本，计算每组大鼠前列腺相对重量。对前列腺组织进行苏木精-伊红（HE）染色后，用光镜观察每组前列腺组织的结构变化及前列腺炎症状态。进行免疫组织化学检测以评估大鼠前列腺组织中三种炎症因子转化生长因子-β1（TGF-β1）、白细胞介素（IL）-6和IL-8的表达。
大鼠前列腺组织HE染色结果显示，与空白组和去势组相比，E0.05+DHT 0.5 mg/kg组和DHT0.15+E0.15 mg/kg组炎症程度显著增加（<0.05）。然而，一旦DHT浓度超过0.5 mg/kg，炎症程度未进一步加重。免疫组织化学染色结果显示，当外源性E浓度恒定时，与空白组相比，E0.05+DHT 0.15 mg/kg组、E0.05+DHT 0.5 mg/kg组和E0.05+DHT 1.5 mg/kg组中TGF-β1和IL-8表达显著增加（<0.05）。在E0.05+DHT 0.15 mg/kg组和E0.05+DHT 0.5 mg/kg组中，与去势组相比，TGF-β1和IL-8表达显著增加（<0.05）。一旦DHT浓度达到0.5 mg/kg，DHT浓度进一步升高未导致TGF-β1或IL-8表达出现任何显著变化。此外，当外源性DHT浓度保持不变时，与空白组和去势组相比，DHT0.15+E 0.05 mg/kg组和DHT0.15+E 0.5 mg/kg组中TGF-β1、IL-6和IL-8表达显著增加（<0.05）。
去势联合不同浓度雌激素和雄激素处理可成功诱导SD大鼠前列腺炎模型。
