Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia.
Veterinary Specialised Institute, Kraljevo, Serbia.
J Vet Diagn Invest. 2022 Jul;34(4):723-726. doi: 10.1177/10406387221102432. Epub 2022 Jun 7.
Although spp. often cause disease in domestic birds (chickens and companion birds), there are few data on avian tuberculosis in wild populations, especially in birds of prey. We describe here a case of a young adult female, free-living Eurasian griffon vulture () that was found dead. Granulomas were grossly evident in the lungs at autopsy, and tuberculosis was suspected. Ziehl-Neelsen staining revealed large numbers of intracellular acid-fast-positive bacteria within granulomas. Examination on Löwenstein-Jensen medium was negative, but mycobacteria growth indicator tube medium results were positive. For the molecular detection of spp., the primer set IS901F and IS901R was used. Positive results were observed on gel electrophoresis, indicating the presence of subsp. DNA. Although tuberculosis is not considered to be a common cause of death in wild birds, it undoubtedly deserves special attention because vultures are generally considered to be a species resistant to a large number of pathogens. Determination of the cause of death of griffon vultures is important for future conservation measures for this sensitive wild species.
虽然 spp. 通常会导致家禽（鸡和伴侣鸟）患病，但有关野生种群，特别是猛禽中的鸟类结核病的数据很少。我们在这里描述了一个年轻的成年雌性自由生活的欧亚鹫（），它被发现死亡。尸检时肺部明显有肉芽肿，怀疑是结核病。齐尔-尼尔森染色显示肉芽肿内有大量细胞内抗酸性阳性细菌。洛温斯坦-詹森培养基检查为阴性，但分枝杆菌生长指示剂管培养基结果为阳性。为了检测 spp.，使用了引物对 IS901F 和 IS901R。凝胶电泳观察到阳性结果，表明存在 亚种 DNA。尽管结核病不被认为是野生鸟类常见的死亡原因，但由于秃鹫通常被认为是一种对大量病原体具有抵抗力的物种，因此它无疑值得特别关注。确定鹫的死因对于这种敏感的野生物种的未来保护措施非常重要。
