Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Av. Professor Egas Moniz, Ed. Egas Moniz, 1649-028 Lisboa, Portugal.
Instituto de Farmacologia e Neurociências, Faculdade de Medicina, Universidade de Lisboa, Av. Professor Egas Moniz, Ed. Egas Moniz, 1649-028 Lisboa, Portugal.
J Mater Chem B. 2022 Jun 22;10(24):4672-4683. doi: 10.1039/d2tb00393g.
Supporting mammalian cells against reactive oxygen species such as hydrogen peroxide (HO) is essential. Bottom-up synthetic biology aims to integrate designed artificial units with mammalian cells. Here, we used manganese dioxide nanosheets (MnO-NSs) as catalytically active entities that have superoxide dismutase-like and catalase-like activities. The integration of these MnO-NSs into 7 μm reactors was able to assist SH-SY5Y neuroblastoma cells when stressed with HO. Complementary, Janus-shaped 800 nm reactors with one hemisphere coated with MnO-NSs showed directed locomotion in cell media with top speeds up to 50 μm s when exposed to 300 mM HO as a fuel, while reactors homogeneously coated with MnO-NSs were not able to outperform Brownian motion. These Janus-shaped reactors were able to remove HO from the media, protecting cells cultured in the proximity. This effort advanced the use of bottom-up synthetic biology concepts in neuroscience.
支持哺乳动物细胞对抗过氧化氢（HO）等活性氧是至关重要的。自下而上的合成生物学旨在将设计的人工单元与哺乳动物细胞集成。在这里，我们使用二氧化锰纳米片（MnO-NSs）作为具有超氧化物歧化酶和过氧化氢酶样活性的催化活性实体。将这些 MnO-NSs 整合到 7 μm 反应器中，当 SH-SY5Y 神经母细胞瘤细胞受到 HO 胁迫时，能够为其提供帮助。此外，具有一个涂有 MnO-NSs 半球的 800nm 的双面 Janus 形反应器在暴露于 300mM HO 作为燃料时，在细胞培养基中的定向运动速度高达 50 μm s，而均匀涂有 MnO-NSs 的反应器则无法超越布朗运动。这些双面 Janus 形反应器能够从培养基中去除 HO，从而保护附近培养的细胞。这项工作推进了自下而上的合成生物学概念在神经科学中的应用。
