Department of Biochemistry, University of Wisconsin-Madison, WI, USA.
FEBS Lett. 2022 Sep;596(17):2269-2287. doi: 10.1002/1873-3468.14420. Epub 2022 Jun 18.
Endocytic trafficking underlies processes essential for plant growth and development, including the perception of and response to abiotic and extracellular stimuli, post-Golgi and exocytic trafficking, and cytokinesis. Protein adaptors and regulatory factors of clathrin-mediated endocytosis that contribute to the formation of endocytic clathrin-coated vesicles are evolutionarily conserved. Yet, work of the last ten years has identified differences between the endocytic mechanisms of plants and Opisthokonts involving the endocytic adaptor TPLATE complex, the requirement of actin during CME, and the function of clathrin-independent endocytosis in the uptake of plant-specific plasma membrane proteins. Here, we review clathrin-mediated and -independent pathways in plants and describe recent advances enabled by new proteomic and imaging methods, and conditional perturbation of endocytosis. In addition, we summarize the formation and trafficking of clathrin-coated vesicles based on temporal and structural data garnered from high-resolution quantitative imaging studies. Finally, new information about the cross-talk between endocytosis and other endomembrane trafficking pathways and organelles will also be discussed.
内吞作用是植物生长和发育所必需的过程的基础，包括对非生物和细胞外刺激的感知和反应、高尔基体后和胞吐作用以及胞质分裂。网格蛋白介导的内吞作用的蛋白质衔接子和调节因子有助于内吞作用的网格蛋白包被囊泡的形成，在进化上是保守的。然而，过去十年的工作已经确定了植物和后生动物之间的内吞作用机制之间的差异，涉及内吞作用衔接子 TPLATE 复合物、CME 期间肌动蛋白的需求以及非网格蛋白依赖性内吞作用在植物特异性质膜蛋白摄取中的功能。在这里，我们综述了植物中的网格蛋白介导的和非网格蛋白依赖性途径，并描述了新的蛋白质组学和成像方法以及内吞作用的条件性扰动所带来的最新进展。此外，我们根据来自高分辨率定量成像研究的时间和结构数据总结了网格蛋白包被囊泡的形成和运输。最后，还将讨论内吞作用与其他内膜运输途径和细胞器之间的交流的新信息。
