Department of Medical, Oral and Biotechnological Sciences, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy.
Orofacial Development and Regeneration, Institute of Oral Biology, University of Zurich, Zurich, Switzerland.
Methods Mol Biol. 2022;2472:197-208. doi: 10.1007/978-1-0716-2201-8_16.
Exosomes are extracellular vesicles involved in cell-to-cell communication as well as extrusion of biological material. Using dental pulp stem cells culture as a model, we hereby describe a method for the packaging of Delta-like 4 (DLL4), a representative Notch ligand, into newly generated exosomes. We then provide methods of analysis to confirm the presence of Notch proteins and transcripts internalization and transport via exosomes.
外泌体是参与细胞间通讯以及生物物质外排的细胞外囊泡。我们以牙髓干细胞培养为模型，描述了一种将 Delta-like 4（DLL4），一种代表性的 Notch 配体，包装到新生成的外泌体中的方法。然后，我们提供了分析方法来确认 Notch 蛋白和转录本通过外泌体内化和运输的存在。
