《新型隐球菌细胞活力检测方法》

Cell Viability Assays for Candida auris.

机构信息

Oral Sciences Research Group, College of Medical, Veterinary and Life Sciences, Glasgow University, Glasgow, UK.

Glasgow Biofilm Research Network, Glasgow, UK.

出版信息

Methods Mol Biol. 2022;2517:129-153. doi: 10.1007/978-1-0716-2417-3_10.

DOI:10.1007/978-1-0716-2417-3_10

PMID:35674950

Abstract

Cell viability assays are useful for assessing the efficacy of antifungal therapeutics and disinfection strategies in vitro. In recent years these assays have been fundamental for the testing of conventional and novel therapies against the nosocomial fungal pathogen Candida auris. Here we provide detailed descriptions of methods for assessing cellular viability of Candida auris in vitro, such as metabolic assays (XTT and resazurin), colony-forming unit counting, live/dead quantitative PCR, and fluorescent staining for microscopic analyses.

摘要

细胞活力检测法在评估抗真菌治疗和体外消毒策略的疗效方面非常有用。近年来，这些检测法对于测试针对医院真菌病原体耳念珠菌的传统和新型治疗方法至关重要。在这里，我们提供了详细描述体外评估耳念珠菌细胞活力的方法，如代谢检测法（XTT 和 Resazurin）、集落形成单位计数、实时定量 PCR 以及荧光染色进行显微镜分析。