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通过轮廓长度和活性对活性聚合物状蠕虫混合物进行色谱分离。

Chromatographic separation of active polymer-like worm mixtures by contour length and activity.

作者信息

Heeremans Tess, Deblais Antoine, Bonn Daniel, Woutersen Sander

机构信息

Van der Waals-Zeeman Institute, IoP, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, Netherlands.

Van 't Hoff Institute for Molecular Sciences, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, Netherlands.

出版信息

Sci Adv. 2022 Jun 10;8(23):eabj7918. doi: 10.1126/sciadv.abj7918. Epub 2022 Jun 8.

Abstract

The convective transport rate of polymers through confined geometries depends on their size, allowing for size-based separation of polymer mixtures (chromatography). Here, we investigate whether mixtures of active polymers can be separated in a similar manner based on their activity. We use thin, living worms as a model system for active polymers and study the transport of these worms by an imposed flow through a channel filled with a hexagonal pillar array. The transport rate through the channel depends strongly on the degree of activity, an effect that we assign to the different distribution of conformations sampled by the worms depending on their activity. Our results demonstrate a unique way to sort mixtures of active polymers based on their activity and provide a versatile and convenient experimental system to investigate the hydrodynamics of active polymers.

摘要

聚合物通过受限几何结构的对流传输速率取决于其尺寸,这使得基于尺寸的聚合物混合物分离(色谱法)成为可能。在此,我们研究活性聚合物混合物是否能基于其活性以类似方式进行分离。我们使用细长的活体蠕虫作为活性聚合物的模型系统,并通过施加流动使其通过填充有六角柱阵列的通道来研究这些蠕虫的传输。通过通道的传输速率强烈依赖于活性程度,我们将这种效应归因于蠕虫根据其活性对所采样构象的不同分布。我们的结果展示了一种基于活性对活性聚合物混合物进行分类的独特方法,并提供了一个通用且便捷的实验系统来研究活性聚合物的流体动力学。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/55cd/9177071/b19ff823f7e5/sciadv.abj7918-f1.jpg

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