Shaanxi Key Laboratory of Photoelectric Functional Materials and Devices, School of Materials and Chemical Engineering, Xi'an Technological University, Xi'an 710021, People's Republic of China.
School of Chemistry and Chemical Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, People's Republic of China.
Anal Bioanal Chem. 2022 Aug;414(19):5887-5897. doi: 10.1007/s00216-022-04154-9. Epub 2022 Jun 9.
Hypochlorous acid (HClO), the core bactericidal substance of the human immune system, plays a vital role in many physiological and pathological processes in the human body. In this work, we designed and synthesized a novel deep-red fluorescent probe TCF-ClO for the determination of hypochlorous acid through theoretical analysis. The results showed that probe TCF-ClO exhibited excellent characteristics of long-wavelength emission (635 nm), fast response (< 1 min), and low detection limit (24 nM). In addition, it had been successfully used for imaging of HClO in living HeLa cells. More importantly, the TCF-ClO composited paper-based sensing material was successfully constructed. The RGB/gray value was obtained from a mobile phone and computer, which could achieve the quantitative detection of HClO, with a linear detection range of 0-50 μM and a detection limit of 1.09 μM (RGB mode)/3.38 μM (gray mode). The function of the paper-based sensor extended from qualitative to quantitative detection of HClO, and it is expected to become a portable device widely used in the environmental area.
次氯酸（HClO）是人体免疫系统的核心杀菌物质，在人体的许多生理和病理过程中都起着至关重要的作用。在这项工作中，我们通过理论分析设计并合成了一种新型的深红色荧光探针 TCF-ClO，用于测定次氯酸。结果表明，探针 TCF-ClO 具有长波长发射（635nm）、快速响应（<1min）和低检测限（24nM）的优异特性。此外，它已成功用于活 HeLa 细胞中 HClO 的成像。更重要的是，成功构建了 TCF-ClO 复合纸质传感材料。通过手机和电脑获得 RGB/灰度值，可以实现 HClO 的定量检测，线性检测范围为 0-50μM，检测限为 1.09μM（RGB 模式）/3.38μM（灰度模式）。纸质传感器的功能从定性检测扩展到 HClO 的定量检测，有望成为一种在环境领域广泛应用的便携式设备。
